HumanIL-1 beta/IL-1 F2 Immuno-PCR Assay Kit, BIQ-ELISA™
The Human Bead-based Immuno-Quantitative (BIQ) IL-1 beta/IL-1 F2 ELISA Kit merges the specificity of sandwich-based ELISA, the sensitivity of real-time PCR, and the easy manipulation of microspheres to detect even the lowest levels of IL-1 beta/IL-1 F2 in serum, plasma, and cell culture supernatant samples.
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
|---|---|
| Species | Human |
| Accession Number | P01584 |
| Gene Id | 3553 |
| Gene Symbols | IL1B, IL1F2 |
| Protein Name / Synonyms | Interleukin-1 beta (IL-1 beta) (Catabolin) |
| Quantitative/Semi-Quantitative | Quantitative |
| Specificity | The IL-1 beta antibodies used in the kit are specific to human IL-1 beta. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Solid Support | 96-well Microplate |
| Method Of Detection | qPCR |
| Design Principle | Sandwich-based |
| Sensitivity | 0.006 pg/ml |
| Detection Range | 0.14 pg/ml - 100 pg/ml |
| Recommended Dilution (Serum/Plasma) | 2- to 10-fold |
| Estimated Lead Time | 3-5 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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Related Products
| Sample Type | Recovery (%) | Range (%) |
|---|---|---|
| Serum | 85 | 76-92 |
| Cell culture media | 103 | 94-109 |
Kit Components
| Component | Size / Qty |
|---|---|
| 96-well V-bottom Plate | 1 plate |
| 96-well Plate Film | 1 plate |
| 96-well PCR Plate | 1 plate |
| 1X Assay Diluent | 1 bottle |
| 20X Wash Buffer Concentrate | 1 bottle |
| Lyophilized Standard | 1 vial |
| Antibody Coated Beads | 1 vial |
| Oligonucleotide Conjugated Antibody | 1 vial |
| 1X Elution Buffer | 1 vial |
| 2X PCR Master Mix | 1 vial |
| Primer Mix | 1 vial |
| PCR Plate Film | 1 piece |
*The PCR plate used is a 0.2 mL, non-skirted 96-well plate (Thermo Fisher, cat. no. AB0600). Please ensure compatibility with your PCR machine prior to purchase. For additional information contact technical support (techsupport@raybiotech.com).
Additional Materials Required
- Real-time PCR instrument, Bio-Rad or QuantStudio™ recommended
- Orbital 96-well plate shaker (with ability to reach 700 rpm)
- Microplate Washer (BioTek 405 TS Washer recommended) and a compatible 96-well V-bottom plate magnetic stand (ThermoFisher AM10050 recommended)
- Precision pipettes to deliver 1 μL to 1 mL volumes
- Pipette tips (barrier tips are recommended to reduce contamination)
- Adjustable 1-25 mL pipettes for reagent preparation
- Centrifuge that can spin 96 well plates at 500 g
- Nuclease-free water or deionized water
- 1.5 mL, 2 mL, or 5 mL microcentrifuge tubes
Assay Procedure Summary
- Prepare all reagents, samples and standards as instructed.
- Add 5 μL of the bead and conjugate mix, and 5 μL of each standard and sample to each well. Incubate for 2.5 hours at room temperature while shaking.
- Add 40 μL Assay Diluent and wash the plate using a Microplate Washer.
- Add 40 μL Elution buffer to each well. Incubate for 10 minutes at room temperature.
- Prepare PCR/Primer Master Mix and pipette 15 μL into each well of the PCR plate.
- Transfer 5 μL supernatant from V-bottom plate to the PCR plate.
- Run real-time PCR.
Storage/Stability
Upon receiving the kit, store 2X PCR Master Mix, Primer Mix and Lyophilized Standard at -20°C. Store other components at 4°C. If stored at the proper temperature, the kit may be stored for up to 12 months from the date of shipment. Avoid repeated freeze-thaw cycles.
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