RayBio® Human IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

As low as $420.00
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Catalog #:
ELH-IL1b
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Product Description

Specifications

Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species Human
Accession Number P01584
Gene Id 3553
Gene Symbols IL1B|IL1F2
Protein Name / Synonyms Interleukin-1 beta (IL-1 beta) (Catabolin)
Quantitative/Semi-Quantitative Quantitative
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample Types Cell Culture Supernatants, Plasma, Serum
Solid Support 96-well Microplate
Method Of Detection Colorimetric
Design Principle Sandwich-based
Research Area Antibody Drug Development, Protein Therapeutics, Biomarker Detection
Sensitivity 0.3 pg/ml

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Detection Range 0.3 pg/ml - 100 pg/ml
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage ≤-20°C

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Target Background

Interleukin-1 beta (IL-1b) plays a critical role in immune and inflammatory responses. It is produced in response to infection, inflammatory agents, and endotoxins. It also plays an important role in bone remodeling, fever, and metabolism of carbohydrates. Increased production of IL-1b is responsible for a variety of autoinflammatory syndromes.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

GCLP Validation

This assay is currently available for GCLP or Good Clinical Laboratory Practice testing service. Please contact technical support at techsupport@raybiotech.com for more details if you are interested in scheduling a GCLP testing service for this assay.

Mause S., Ritzel E., Liehn E., et al. Platelet Microparticles Enhance the Vasoregenerative Potential of Angiogenic Early Outgrowth Cells After Vascular Injury. Circulation . 2010 Aug 3;122(5):495-506. doi: 10.1161/CIRCULATIONAHA.109.909473.
Species:
Human
Sample Type:
Conditioned Media (HUVEC cells in vascular injury model)
Unamuno X, Gómez-Ambrosi J, Ramírez B, Rodríguez A, Becerril S, Valentí V, Moncada R, Silva C, Salvador J, Frühbeck G, Catalán V. NLRP3 inflammasome blockade reduces adipose tissue inflammation and extracellular matrix remodeling. Cell Mol Immunol. 2019 Sep 24. doi: 10.1038/s41423-019-0296-z. Epub ahead of print. PMID: 31551515.
Species:
Human
Sample Type:
Conditioned Media
Ildefonso C., Jaime H., Biswal M., et al. Gene therapy with the caspase activation and recruitment domain reduces the ocular inflammatory response. Mol Ther. 2015 May;23(5):875-84. doi: 10.1038/mt.2015.30.
Species:
Human
Sample Type:
Conditioned Media (Human HEK293T cells and ARPE-19 cells)
Diabate M., Muron P., Garcia E., et al. Escherichia coli ?-hemolysin counteracts the anti-virulence innate immune response triggered by the Rho GTPase activating toxin CNF1 during bacteremia. PLoS Pathog. 2015 Mar 17;11(3):e1004732. doi: 10.1371/journal.ppat.1004732.
Species:
Human
Sample Type:
Conditioned Media (Monocytes treated with CNF1 GTPase activating toxin)
Ansari MA, Dutta S, Veettil MV, Dutta D, Iqbal J, Kumar B, et al. (2015) Herpesvirus Genome Recognition Induced Acetylation of Nuclear IFI16 Is Essential for Its Cytoplasmic Translocation, Inflammasome and IFN-? Responses. PLoS Pathog 11(7): e1005019. doi:10.1371/journal.ppat.1005019
Species:
Human
Sample Type:
Conditioned Media (Lots of cell types, dermal endothelial, foreskin fibroblasts and others studied)
Jiang H., Ling Z., Zhang Y., et al. Altered fecal microbiota composition in patients with major depressive disorder. Brain Behav Immun. 2015 Aug;48:186-94. doi: 10.1016/j.bbi.2015.03.016
Species:
Human
Sample Type:
Serum (Major Depressive Disorder Patients)
Chen, Xia, et al. "Lanthanum chloride inhibits LPS mediated expressions of pro-inflammatory cytokines and adhesion molecules in HUVECs: involvement of NF-?B-Jmjd3 signaling." Cellular Physiology and Biochemistry 42.5 (2017): 1713-1724.
Species:
Human
Sample Type:
Conditioned Media
Mankad D., Dupuis A., Smile S., et al. A randomized, placebo controlled trial of omega-3 fatty acids in the treatment of young children with autism. Mol Autism. 2015; 6: 18. Published online 2015 Mar 21. doi: 10.1186/s13229-015-0010-7
Species:
Human
Sample Type:
Plasma (austistic children treatment with omega 3 fatty acids)
Scherer O, Steinmetz H, Kaether C, Weinigel C, Barz D,Kleinert H, Menche D, M¨uller R, Pergola C, Werz O, Targeting V-ATPase in primaryhuman monocytes by archazolid potently represses the classical secretion of cytokinesdue to accumulation at the endoplasmic reticulum, Biochemical Pharmacology (2014), http://dx.doi.org/10.1016/j.bcp.2014.07.028
Species:
Human
Sample Type:
Cell Lysate (Human monocyte lysates)
Alkhatib B., et al. ACUTE MECHANICAL INJURY OF THE HUMAN INTERVERTEBRAL DISC: LINK TO DEGENERATION AND PAIN. European Cells and Materials, 2014 (28) 98-111.
Species:
Human
Sample Type:
Conditioned Media (Vertebral disc conditioned media)
  1. Great product
    Rating
    100%
    We have purchased this kit on multiple occasions to detect IL-1B in human tissue effluent samples. Kit is very reasonably priced, and with good technique has very repeatable results. Highly recommend!

    Review by

    from N/A,

    on

  2. Good
    Rating
    100%
    Easy to use and reliable results

    Review by

    on

  3. Great product
    Rating
    100%
    This kit worked very will with human serum samples. Great sensitivity!

    Review by

    from BCM,

    on

  4. Great product
    Rating
    100%
    This ELISA kit was used for quantification of IL-1 beta cytokine in the supernatants of peripheral blood mononuclear cells upon stimulation with genotoxics in a dose- and time dependent manner. The optical density (O.D.) values obtained were repetitive.

    Review by

    from KYNAN PHARMA,

    on

  5. Great product
    Rating
    100%
    Used cell culture sample type. It is not as costly as other kits but requires more labor. Results will be as consistent as the user's technique. If you have previous experience with ELISA you will have no problems, if you are new to ELISA it may be more difficult.

    Review by

    from NanoBST,

    on

  6. Great product
    Rating
    100%
    Good performance. The range provided for the IL-1beta cytokine (0.3-100 pg/ml) analysis is very consistent with the results. I'm thinking of buying another kit from Raybio.

    Review by

    from University of Texas Dallas,

    on

  7. Great product
    Rating
    100%
    Worked well for cell culture supernatant.

    Review by

    from University of South Florida,

    on

  8. Great product
    Rating
    100%
    I bought the human IL1b ELISA kit in October, 2018. It's perfect for cell culture supernatants.

    Review by

    from Augusta University,

    on

  9. Great product
    Rating
    100%
    Excellent

    Review by

    from University of the Pacific,

    on

  10. Great product
    Rating
    100%
    I used this kit on conditioned media from cardiac progenitor cells. I didn't dilute my samples and it worked very well.

    Review by

    from Emory University,

    on

  11. Great product
    Rating
    100%
    I used it with human corneal epithelial cell supernatant treated with various stimulus. It's very important for me to have high quality and sensitivity. This product provides that. Also very easy to use and follow the instructions on the protocol.

    Review by

    from University of Illinois at Chicago,

    on

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