Quantibody® Human Th17 Array 1 Kit. Detects 20 Human Th1, Th2, and Th17 Cytokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 20 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
GM-CSF
| IFN-gamma
| IL-1 beta (IL-1 F2)
| IL-10
| IL-12 p70
|
IL-13
| IL-17A
| IL-17F
| IL-2
| IL-21
|
IL-22
| IL-23
| IL-28A (IFN-lambda 2)
| IL-4
| IL-5
|
IL-6
| MIP-3 alpha (CCL20)
| TGF beta 1
| TNF alpha
| TNF beta (TNFSF1B)
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Th1/Th2/Th17 Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Yang P., Foster CS. Interleukin 21, interleukin 23, and transforming growth factor ?1 in HLA-A29-associated birdshot retinochoroidopathy. Am J Ophthalmol. 2013 Aug;156(2):400-406.e2. doi: 10.1016/j.ajo.2013.03.004.
Species:
Human
Sample Type:
Serum (Birdshot Retinochoroidopathy samples)
Stechova K., Halbhuber Z., Hubackova M., et al. Case report: type 1 diabetes in monozygotic quadruplets. Eur J Hum Genet . 2012 Apr;20(4):457-62. doi: 10.1038/ejhg.2011.212.
Species:
Human
Sample Type:
Conditioned Media (PBMCs from Diabetic and non diabetic patients)
Liu R, Wu Q, Su D, et al. A regulatory effect of IL-21 on T follicular helper-like cell and B cell in rheumatoid arthritis. Arthritis Research & Therapy. 2012;14(6):R255. doi:10.1186/ar4100.
Species:
Human
Sample Type:
Serum (Rheumatoid Arthritis and Healthy control samples)
Celis R., et al. Synovial cytokine expression in psoriatic arthritis and associations with lymphoid neogenesis and clinical features. Celis et al. Arthritis Research & Therapy 2012, 14:R93
Species:
Human
Sample Type:
Synovial Fluid
Gomez-Puerta J., et al. Differences in synovial fluid cytokine levels but not in synovial tissue cell infiltrate between anti-citrullinated peptide/protein antibody-positive and -negative rheumatoid arthritis patients. Gómez-Puerta et al. Arthritis Research & Therapy 2013, 15:R182
Species:
Human
Sample Type:
Synovial Fluid
Canete J., Celis R., Yeremenko N., et al. Ectopic lymphoid neogenesis is strongly associated with activation of the IL-23 pathway in rheumatoid synovitis. Arthritis Res Ther. 2015 Jul 9;17:173. doi: 10.1186/s13075-015-0688-0.
Species:
Human
Sample Type:
Synovial Fluid (Synoival fluid from sites of rheumatoid synovitis)
Kotlarz D, Zi?tara N, Uzel G, et al. Loss-of-function mutations in the IL-21 receptor gene cause a primary immunodeficiency syndrome. J. Exp. Med. 2013 Mar 11;210(3):433-443.
Species:
Human
Sample Type:
Conditioned Media ( PBMC cells)
Du X., Shi R., Wang Y., et al. Isoforskolin and forskolin attenuate lipopolysaccharide-induced inflammation through TLR4/MyD88/NF-?B cascades in human mononuclear leukocytes. Phytother Res. 2019 Jan 14. doi: 10.1002/ptr.6248.
Species:
Human
Sample Type:
Conditioned Media (mononuclear leukocytes treated with various isoforskolin and forskolin treatments)
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