Quantibody® Human Matrix Metalloproteinase Antibody Array 1 Kit. Detects 10 Human MMPs. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 10 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
MMP-1
| MMP-10
| MMP-13
| MMP-2
| MMP-3
|
MMP-8
| MMP-9
| TIMP-1
| TIMP-2
| TIMP-4
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human MMP Array Q1 Slide(s)
- Sample Diluent
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
RodrÃÂguez?Muñoz, Y., et al. "Peripheral blood monocyte subsets predict antiviral response in chronic hepatitis C." Alimentary pharmacology & therapeutics 34.8 (2011): 960-971.
Species:
Human
Sample Type:
Conditioned Media (peripheral monocytes )
Sava P., Ramanathan A., Dobronyi A., et al. Human pericytes adopt myofibroblast properties in the microenvironment of the IPF lung. JCI Insight. 2017 Dec 21;2(24). pii: 96352. doi: 10.1172/jci.insight.96352
Species:
Human
Sample Type:
Conditioned Media (Human Pericytes)
Martin G., Asensi V., Montes H., Collazos J., Alvarez V., Carton J., Taboada F., Valle-Garay E. Role of plasma matrix-metalloproteases (MMPs) and their polymorphisms (SNPs) in sepsis development and outcome in ICU patients. Scientific Reports 4, 5002. doi:10.1038/srep05002.
Species:
Human
Sample Type:
Plasma
Fan X, Brezski RJ, Fa M, et al. A single proteolytic cleavage within the lower hinge of trastuzumab reduces immune effector function and in vivo efficacy. Breast Cancer Research?: BCR 2012;14(4):R116. doi:10.1186/bcr3240.
Species:
Human
Sample Type:
Tissue Lysate (Breast cancer tissue vs adjacent tissue)
Tian F., Qin C., Li X., et al. Decreased stathmin-1 expression inhibits trophoblast proliferation and invasion and is associated with recurrent miscarriage. Am J Pathol. 2015 Oct;185(10):2709-21. doi: 10.1016/j.ajpath.2015.06.010.
Species:
Human
Sample Type:
Conditioned Media (HTR-8 cells treated with siRNAs for Stathmin-1, invasive extravillous trophoblasts)
Suarez-Zarracina T., Valle-Garay E., Collazos J., et al. Didanosine (ddI) associates with increased liver fibrosis in adult HIV-HCV coinfected patients. J Viral Hepat. 2012 Oct;19(10):685-93. doi: 10.1111/j.1365-2893.2012.01596.x
Species:
Human
Sample Type:
Serum (HIV and HCV coinfected patients with liver fibrosis)
Epanchintsev A., Pavithra S., Verma R., Lyakhovich A. IL-6, IL-8, MMP-2, MMP-9 are overexpressed in Fanconi anemia cells through a NF-?B/TNF-? dependent mechanism
Species:
Human
Sample Type:
Conditioned Media (MDA-MB-231, PC3, and MCF7 cancer cells)
Collazos J., Asensi V., Martin G., et al. The effect of gender and genetic polymorphisms on matrix metalloprotease (MMP) and tissue inhibitor (TIMP) plasma levels in different infectious and non-infectious conditions. Clin Exp Immunol. 2015 Nov;182(2):213-9. doi: 10.1111/cei.12686.
Species:
Human
Sample Type:
Plasma (Gender and infectious vs non infectious conditioned patients)
Cekanova M., Feranando RI., Siriwardhana N., et al. BCL-2 family protein, BAD is down-regulated in breast cancer and inhibits cell invasion. Exp Cell Res. 2015 Feb 1;331(1):1-10. doi: 10.1016/j.yexcr.2014.11.016.
Species:
Human
Sample Type:
Cell Lysate (MCF7 cell lysates following BAD induction)
Cekanova M., Fernando R., Siriwardhana N., et al. BCL-2 family protein, BAD is down-regulated in breast cancer and inhibits cell invasion. Exp Cell Res. 2015 Feb 1;331(1):1-10. doi: 10.1016/j.yexcr.2014.11.016.
Species:
Human
Sample Type:
Conditioned Media (Human MCF-7 cells silenced with BAD siRNA)
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Great Product and Customer ServiceWorked great with conditioned media from porcine tissue. Customer service was very helpful regarding technical questions, and when it came to data analysis, they were immensely helpful.
from Clemson University,
on
Great productThis array, like their other Q-type products, worked well, was easy to use, and provided a lot of data for my samples at a great cost relative to competitors. I successfully ran supernatants from functional engineered bone tissues and cancer cell lines. The data analysis by the manufacturer was quick as always. As someone with expertise in cancer cell and engineered tissue culture, I would highly recommend this product.from Columbia University,
on
Great productThe end user in our lab had the following to say: "I would rate it 5/5. Works as described. Affordable."from Hershey Medical Center,
on
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