RayBio® C-Series Human Inflammation Antibody Array 1 Kit. Detects 20 Human Inflammatory Factors. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 20 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Membrane |
| Method Of Detection | Chemiluminescence |
| Design Principle | Sandwich-based |
| Research Area | Inflammation |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| GCSF
| GM-CSF
| IFN-gamma
|
IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-2
| IL-3
| IL-4
|
IL-6
| IL-7
| IL-8 (CXCL8)
| IL-10
| IL-11
|
IL-12 p40
| IL-12 p70
| IL-13
| I-309 (TCA-3/CCL1)
| TIMP-2
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Inflammation Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Burkholder K., Bhunia A. Salmonella enterica serovar Typhimurium adhesion and cytotoxicity during epithelial cell stress is reduced by Lactobacillus rhamnosus GG. Gut Pathog. 2009 Jul 9;1(1):14. doi: 10.1186/1757-4749-1-14.
Species:
Human
Sample Type:
Conditioned Media (Caco2 cells treated or untreated with S. Typhuimurium)
Tonade, Deoye, et al. "Photoreceptor cells produce inflammatory products that contribute to retinal vascular permeability in a mouse model of diabetes." Diabetologia 60.10 (2017): 2111-2120.
Species:
mouse
Sample Type:
Conditioned Media (retinal endothelial cells)
Fernandez A M, Fernandez S, Carrero P, Garcia-Garcia M, Torres-Aleman I. Calcineurin in Reactive Astrocytes Plays a Key Role in the Interplay between Proinflammatory and Anti-Inflammatory Signals.J Neuroscience.2007;27(33):8745-8756
Species:
Human
Sample Type:
Cell Lysate (AIC astrocyte culture Lysate)
Schriener B, Voss J, Wischhusen J, Dombrowski Y, et al. Expressin of toll-like receptors by human muscle cells in vitro and in vivo: TLR3 is highly expressed in inflammatory and HIV myopathies, mediates IL-8 release and up-regulation of NKG2D ligands. FASEB J. 2005;19(13):U645-U666.
Species:
Human
Sample Type:
Conditioned Media (rhabdomyosarcoma (TE671) and myoblast Cell-conditioned media)
Zhao L., Yagiz Y., Xu C., et al. Muscadine grape seed oil as a novel source of tocotrienols to reduce adipogenesis and adipocyte inflammation. Food Funct. 2015 Jul;6(7):2293-302. doi: 10.1039/c5fo00261c.
Species:
Human
Sample Type:
Conditioned Media (Adipose derived stem cells treated with muscadine grape seed oil)
Nakamura Y, Nawata M, Wakitani S. Expression Profiles and Functional Analyses of Wnt-Related Genes in Human Joint Disorders. Am J Pathol. Jul 2005; 167(1): 97-105.
Species:
Human
Sample Type:
Conditioned Media (Conditioned Media)
Maldonado, R., et al. "Cytotoxic effects of Kingella kingae outer membrane vesicles on human cells." Microbial pathogenesis 51.1 (2011): 22-30.
Species:
Human
Sample Type:
Conditioned Media
Sanchez-Quesada C., Lopez-Biedma A., Gaforio J. Maslinic Acid enhances signals for the recruitment of macrophages and their differentiation to m1 state. Evid Based Complement Alternat Med. 2015;2015:654721. doi: 10.1155/2015/654721.
Species:
Human
Sample Type:
Conditioned Media (THP1 human macrophages stimulated with LPS)
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