Human Immune Checkpoint Array C1
Membrane-based immune-checkpoint cytokine array detects 23 human molecules expressed in T/B cells and APCs.
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 23 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Membrane |
| Method Of Detection | Chemiluminescence |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
4-1BB (TNFRSF9/CD137)
| B7-H2 (ICOS Ligand)
| B7-H3 (CD276)
| B7-H4
| CD200
|
CD200 R1
| CD27 (TNFRSF7)
| HVEM (TNFRSF14/CD270)
| CD27 Ligand (TNFSF7)
| CD28
|
CD40 Ligand (TNFSF5)
| CD80 (B7-1)
| CD86 (B7-2)
| CTLA-4 (CD152)
| ICOS
|
LAG-3
| OX40 (TNFRSF4)
| OX40 Ligand (TNFSF4)
| PD-1
| B7-H1 (CD274/PD-L1)
|
PD-L2 (B7-DC)
| TIM-1 (KIM-1)
| TIM-3 (KIM-3)
| ||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Immune Checkpoint Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
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Work perfectlyI used this kit to detect human immune checkpoints in the serum of healthy donors and cancer patients. The kit is user-friendly, providing clear and specific signals. It was very helpful for my initial screening and provided valuable insights for my further study.
from The University of Texas MD Anderson Cancer Center,
on
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