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    Human B7H1/PD-L1 ELISA Kit

    Rating:
    93 % of 100
    3  Reviews

    RayBio® Human B7-H1 (CD274) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

    Lead time: Typically ships within 1-2 business days. No Friday shipments.

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    As low as $500.00
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    Catalog #:
    ELH-B7H1
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    Product Description

    Specifications

    Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
    Species Human
    Accession Number Q9NZQ7
    Gene Id 29126
    Gene Symbols CD274|B7H1|PDCD1L1|PDCD1LG1|PDL1
    Protein Name / Synonyms Programmed cell death 1 ligand 1 (PD-L1) (PDCD1 ligand 1) (Programmed death ligand 1) (B7 homolog 1) (B7-H1) (CD antigen CD274)
    Quantitative/Semi-Quantitative Quantitative
    Specificity The antibody pair provided in this kit recognizes Human B7-H1 (CD274)
    Compatible Sample Types Cell Culture Supernatants, Plasma, Serum
    Solid Support 96-well Microplate
    Method Of Detection Colorimetric
    Design Principle Sandwich-based
    Research Area Antibody Drug Development, Protein Therapeutics, Biomarker Detection
    Sensitivity 46 pg/ml

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    Detection Range 46 pg/ml - 3000 pg/ml
    Recommended Dilution (Serum/Plasma) 2 fold
    Estimated Lead Time 1-2 business days
    Shipping Type Blue ice
    Storage ≤-20°C

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    Product Features

    Additional Notes
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Standard Curves

    Spiking & Recovery Results

    Linearity Results

    Application Notes

    Kit Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Other Materials Required
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Protocol Outline
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.

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    Storage/Stability

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Reviews

    Write a customer review
    1. Great product
      Rating
      100%
      I am using the kits from RayBiotech and it easy to follow with the cell culture supernatant. You might need to try different fold of dilutions.

      Review by

      from Florida A&M University,

      on

    2. Great product
      Rating
      100%
      Great kit. Protocol easy to follow, as all other ELISA protocols from RayBiotech. Great and reproducible results.

      Review by

      from Florida A&M University,

      on

    3. Great product
      Rating
      80%
      Kit works very well. Nice, reproducible results. The instructions can be a little confusing (example "Assay Diluent B" is labeled "Item E" and "Diluent D" is "Item K"), but as long as you read carefully, shouldn't be a problem.

      Review by

      from Emory University - SOM: PEDS,

      on

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