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Human Cytokine Array Q640
Quantibody® Human Cytokine Antibody Array 640 Kit. A combination of 16 non-overlapping arrays to quantitatively measure 640 human cytokines. Suitable for all liquid sample types.
Product Description
Specifications
Size
8 Sample Kit, 22 Sample Kit, 50 Sample Kit
Species
Human
Quantitative/Semi-Quantitative
Quantitative
Number of Targets Detected
640
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates
Solid Support
Glass Slide
Method Of Detection
Fluorescence Laser Scanner
Design Principle
Sandwich-based
Estimated Lead Time
1-2 business days
Shipping Type
Blue ice
Storage
-20°C
Amazon Gift Cards!
$5 Amazon gift card in every kit box purchased.
Product Features
More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 800 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation
Target Names
2B4 (CD244), 4-1BB Ligand (TNFSF9), 4-1BB (TNFRSF9/CD137), 6Ckine (CCL21), ACE, ACE-2, Activin A, Activin R1B (ALK-4), Activin R2B (ACVR2B), ADAM-12, ADAM23, ADAM-8, ADAM-9, ADAMTS-13, Adiponectin (ACRP30), Adipsin (Complement Factor D), ADP-Ribosyl Cyclase 2 (BST-1/CD157), aFGF (FGF-1), Aggrecan, AgRP, Albumin, ALCAM (CD166), ALK-1, ALK-6 (BMPR-IB), Alpha-fetoprotein, AMICA, AmiGO, Aminopeptidase LRAP (ERAP2), Aminopeptidase P2 (XPNPEP2), Amnionless, Amphiregulin, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiopoietin-4, Angiostatin, Angiotensinogen (SERPINA8), ANGPTL3, ANGPTL4, APRIL, Artemin, Arylsulfatase A (ARSA), AXL, B7-H1 (CD274), B7-H2 (ICOS Ligand), B7-H3 (CD276), BAFF, BAFF R, BAMBI (NMA), BCAM, bcl-w, BCMA, BDNF, beta IG-H3, Beta-2 Microglobulin, Betacellulin (BTC), beta-NGF, bFGF, BLAME (SLAMF8), BLC (CXCL13), BMP-2, BMP-4, BMP-5, BMP-7, BMP-8, BMP-9, BMPR-IA (ALK-3), BMPR-II, BOC (Brother of CDO), Brevican, C5a, CA12, CA125, CA15-3, CA19-9, CA9, Cadherin-11, Cadherin-13, Cadherin-4, Calcitonin, Calsyntenin-1 (Alcadein-alpha), Carboxypeptidase A2 (CPA2), Cardiotrophin-1 (CT-1), Cathepsin B (Pro-Cathepsin B), Cathepsin E, Cathepsin L, Cathepsin S, Cathepsin V (Cathepsin L2), CCL28 (MEC), CD109, CD14, CD147 (EMMPRIN), CD155 (PVR), CD163, CD200, CD229 (SLAMF3), CD23, CD26 (DPPIV), CD27 (TNFRSF7), CD28, CD30 (TNFRSF8), CD300c (CLM-6), CD320 (TCblR/8D6A), CD34, CD40 Ligand (TNFSF5), CD40 (TNFRSF5), CD48 (SLAMF2), CD58 (LFA-3), CD6, CD80 (B7-1), CD83, CD84 (SLAMF5), CD86 (B7-2), CD97, CD99, CDNF, CDO, CEA (CEACAM-5), CEA (CEACAM-5), CEACAM-1, Chemerin, CHI3L1, CHL-1 (L1CAM-2), CHST1 (GST-1), CHST4 (GST-3), cIAP-2, CILP-1, Ck beta 8-1 (CCL23/MPIF-1), CLEC-1, CLEC10A (CD301), Clusterin, CMG-2 (ANTXR2), CNTF, CNTF R alpha, Coagulation Factor VII, Coagulation Factor XIV (Protein C), Common beta Chain, Complement MASP3, Contactin-1, Contactin-2 (TAG1), CREG, CRIM1, Cripto-1, CRP (C-Reactive Protein), CRTAC1, CRTAM, CTACK (CCL27), CTLA-4 (CD152), CXADR, CXCL14 (BRAK), CXCL16, Cystatin A, Cystatin B, Cystatin C, Cystatin E/M, Cystatin SN, Cytokeratin 8 (KRT8), DAN, DcR3, Decorin, Dectin-1 (CLEC7A), Desmocollin-3, Desmoglein-1, Desmoglein-2, Desmoglein-3, DKK-1, DKK-3, DKK-4, DLL1, DNAM-1 (CD226), Dopa Decarboxylase (DDC), DPPII (QPP/DPP7), DR3 (TNFRSF25), DR6 (TNFRSF21), DSPG3, Dtk, E-Cadherin, EDA-A2, EDAR, EGF, EGFR, EG-VEGF (PK1), EMR2, ENA-78 (CXCL5), Endocan, Endoglin (CD105), Endoglycan (PODXL2), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), EphA1, EphA2, EphB4, EphB6, Ephrin-A4, Ephrin-B3, Epiregulin, ErbB2, ErbB3, ErbB4 (HER4), Erythropoietin R, ESAM, E-Selectin, FABP1, FABP2, FAP, Fas Ligand (TNFSF6), Fas (TNFRSF6/Apo-1), Fc gamma RIIB/C (CD32B/C), FCAR (CD89), FCRL1 (FcRH1), FCRL2 (FcRH2), Ferritin, Fetuin A, FGF-12, FGF-17, FGF-19, FGF-20, FGF-21, FGF-23, FGF-4, FGF-5, FGF-6, FGF-7 (KGF), FGF-9, FLRG, Flt-3 Ligand, Flt-3 (Flk-2), FOLH1 (PSMA), Follistatin, Follistatin-like 1, FOLR1, FOLR2, Fractalkine (CX3CL1), FSH, Furin, Galectin-1, Galectin-2, Galectin-3, Galectin-4, Galectin-7, Galectin-8, Galectin-9, Gas 1, Gas 6, GASP-1 (WFIKKNRP), GASP-2 (WFIKKN), GCP-2 (CXCL6), GCSF, GCSF R (CD114), GDF-15, GDF-8 (Myostatin), GDNF, GITR Ligand (TNFSF18), GITR (TNFRSF18), GLP-1, Glypican 1, Glypican 2, Glypican 5, GM-CSF, GM-CSF R alpha, GOLM1 (GP73), gp130 (sgp130), GPR56, GPVI, Granulysin (NKG5/LAG-2), GRO alpha/beta/gamma, GRO alpha (CXCL1), Growth Hormone, Growth Hormone R, HAI-1, HAI-2, HAPLN1, HB-EGF, HCC-1 (CCL14), HCC-4 (CCL16), hCG beta, Hepsin, HGF, HGFR, HTRA2, HVEM (TNFRSF14), I-309 (TCA-3/CCL1), ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), ICOS, IFN-alpha / beta R2, IFN-gamma, IFN-gamma R1, IGF-1, IGF-1 R, IGF-2, IGF-2 R, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 R1, IL-1 R2, IL-1 R3 (IL-1 R Acp), IL-1 R4 (ST2), IL-1 R5 (IL-18 R alpha), IL-1 R6 (IL-1 Rrp2), IL-1 Ra (IL-1 F3), IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-13 R alpha 1 (IL-13 R1), IL-13 R alpha 2, IL-15, IL-15 R alpha, IL-16, IL-17 RA, IL-17 RB, IL-17A, IL-17B, IL-17C, IL-17E (IL-25), IL-17F, IL-18, IL-18 BP alpha, IL-18 R beta (AcPL), IL-2, IL-2 R alpha, IL-2 R beta (CD122), IL-2 R gamma (Common gamma Chain), IL-20, IL-20 R alpha, IL-21, IL-21 R, IL-22 R alpha 1, IL-22BP, IL-23, IL-23 R, IL-24, IL-27, IL-27 R alpha, IL-28A (IFN-lambda 2), IL-29 (IFN-lambda 1), IL-3, IL-31, IL-31 RA, IL-32 alpha, IL-33 (IL-1 F11), IL-34, IL-36 alpha (IL-1 F6), IL-36 beta (IL-1 F8), IL-36 gamma (IL-1 F9), IL-36 Ra (IL-1 F5), IL-37 (IL-1 F7), IL-38 (IL-1 F10), IL-4, IL-4 R alpha, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-7 R alpha (CD127), IL-8 (CXCL8), IL-9, ILT-2 (LIR-1/CD85j), Insulin, Insulin R (CD220), Integrin alpha 5, IP-10 (CXCL10), I-TAC (CXCL11), Jagged 1, Jagged 2, JAM-A (CD321/F11R), JAM-B (VE-JAM/CD322), JAM-C (CD323), Kallikrein 14, Kallikrein 5, Kallikrein 7, KIRREL3 (NEPH2), KLF4, LAG-3, LAIR1, LAMP (LSAMP), LAMP1 (CD107a), Langerin (CD207), LAP/TGF beta 1 (TGF beta 1), Layilin, LDL R, Legumain, Leptin, Leptin R, LIF, LIF R alpha, LIGHT (TNFSF14), LIMPII, Lipocalin-1, Lipocalin-2 (NGAL), LOX-1, LRIG3, LRP-6, L-Selectin (CD62L), Lumican, Luteinizing hormone, Lymphotactin (XCL1), Lymphotoxin beta R (TNF RIII), LYVE-1, Marapsin, Matrilin-3, Matriptase (ST14), MBL, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, M-CSF R, MDC (CCL22), MDGA1, MDM2, MEPE (OF45), Meprin alpha, Meprin beta (MEP1B), Mer, Mesothelin, MFRP, MICA, MICB, Midkine, MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MIS RII (AMHR2), MMP-1, MMP-10, MMP-13, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MPIF-1 (CCL23), MSP alpha/beta, MSP Receptor (Ron), NAP-2 (PPBP/CXCL7), N-Cadherin (Cadherin-2), NCAM-1 (CD56), Nectin-1, Nectin-3, Nectin-4, Neprilysin, Neprilysin-2 (MMEL1), Neurexin-3 beta (NRXN3), Neuropilin-2, Neurturin, NGFR (TNFRSF16), Nidogen-1, Nidogen-2, NKp30, NKp44, Nogo Receptor (Reticulon-4 Receptor), Notch-1, Notch-3, NOV (CCN3), NrCAM, NRG1-beta 1 (HRG1-beta 1), NSE, NT-3, NT-4, Olfactomedin-2 (Noelin-2), Oncostatin M, Oncostatin M R beta (IL-31 R beta), Osteoactivin (GPNMB), Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), OX40 Ligand (TNFSF4), OX40 (TNFRSF4), p27/Kip1 (CDKN1B), p53, PAI-1, PAPP-A (htPAPP-A), PAPP-A2, PARC (CCL18), P-Cadherin, PD-1, PD-ECGF (Thymidine Phosphorylase), PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-C, PECAM-1, Pentraxin-3 (TSG-14), Pepsinogen 1, Pepsinogen 2, Periostin, Persephin, PGRPs, Platelet Factor 4 (CXCL4), Plexin B3, Plexin D1, PLGF, Pref-1 (DLK-1/FA1), Presenilin 1, Procalcitonin, Progranulin, ProGRP, Prolactin, Prolactin R, Prostasin, PSA-free, PSA-total, PTH, PYY, RAGE, RANK (TNFRSF11A), RANTES (CCL5), RBP4, Reg1B, RELT (TNFRSF19L), Renin, Resistin, Ret, RGM-A, RGM-B, ROBO2, ROBO3, ROBO4, Ryk, S100 A8, SCF, SCF R (CD117/c-kit), SDF-1 alpha (CXCL12 alpha), SDF-1 beta (CXCL12 beta), Semaphorin 6B, Semaphorin 6D, Semaphorin 7A, SERPINA4, SERPINF1 (PEDF), sFRP-3, SIGIRR, Siglec-10, Siglec-11, Siglec-5 (CD170), Siglec-7 (CD328), Siglec-9, SLAM (CD150), Sonic Hedgehog N-Terminal (Shh-N), SOST, SOX2, SP-D, Spinesin, SREC-I (SCARF1), SREC-II (SCARF2), Syndecan-1, Syndecan-3, Syndecan-4, TACE, TACI (TNFRSF13B), TARC (CCL17), TECK (CCL25), Testican 2 (SPOCK2), TFF3 (Trefoil Factor 3), TFPI, TFPI-2, TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, TGF beta R3, Thrombomodulin, Thrombopoietin (TPO), Thrombospondin 1, Thrombospondin 2, Thrombospondin 5 (COMP), Thyroglobulin, Tie-1, Tie-2, TIM-1 (KIM-1), TIM-3 (KIM-3), TIMP-1, TIMP-2, TIMP-4, TLR1, TLR2, TLR3, TLR4, TNF alpha, TNF beta (TNFSF1B), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TRACP (PAP/ACP5), TRAIL R1 (TNFRSF10A/DR4), TRAIL R2 (TNFRSF10B/DR5), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), TRAIL (TNFSF10), TRANCE (TNFSF11), Transferrin, Transferrin, Trappin-2, TREM-1, TREM-2, Tripeptidyl-peptidase I (TPP1), TRKC, TROP1 (EpCAM), Troponin I, TROY (TNFRSF19), TSH, TSLP, TWEAK R (TNFRSF12), TWEAK (TNFSF12), Ubiquitin+1, ULBP-1, ULBP-2 (ALCAN-alpha/N2DL2), ULBP-3, uPA, uPAR, Uromodulin, VCAM-1 (CD106), VE-Cadherin (CDH5), VEGF-A, VEGF-C, VEGF-D, VEGFR1, VEGFR2, VEGFR3, WIF-1, WISP-1 (CCN4), XEDAR, XIAP (BIRC4/IAP-3)
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
Human Cytokine Antibody Array Q640 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual
Other Materials Required
Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays!
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Protocol Outline
Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20° C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20° C and all other reagents undiluted at 4° C for no more than 3 months.
Canestrari E, Steidinger HR, McSwain B, Charlebois SJ, Dann CT. Human Platelet Lysate Media Supplement Supports Lentiviral Transduction and Expansion of Human T Lymphocytes While Maintaining Memory Phenotype. J Immunol Res. 2019 Sep 4;2019:3616120. doi: 10.1155/2019/3616120. PMID: 31565660; PMCID: PMC6746159.
Liu J, Li J, Xie C, Xuan L, Tang B. MSCs attenuate hypoxia induced pulmonary hypertension by activating P53 and NF-kB signaling pathway through TNF? secretion. Biochem Biophys Res Commun. 2020 Nov 12;532(3):400-405. doi: 10.1016/j.bbrc.2020.08.064. Epub 2020 Aug 30. PMID: 32878707.
Sample Type:
Conditioned Media (MSCs)
O D, Waelkens E, Vanhie A, Peterse D, Fassbender A, D'Hooghe T. The Use of Antibody Arrays in the Discovery of New Plasma Biomarkers for Endometriosis. Reprod Sci. 2020 Feb;27(2):751-762. doi: 10.1007/s43032-019-00081-w. Epub 2020 Feb 3. PMID: 32016799.
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