Semi-quantitative, sandwich-based, membrane cytokine array to detect 20 human angiogenesis protein biomarkers.
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 20 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Membrane |
| Method Of Detection | Chemiluminescence |
| Design Principle | Sandwich-based |
| Research Area | Angiogenesis |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
Risk-Free Guarantee
We offer a 100% guarantee on all ELISA kits and membrane cytokine arrays.
Learn More
Amazon Gift Cards!
$5 Amazon gift card in every kit box purchased.
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
|
Angiogenin
|
EGF
|
ENA-78
(CXCL5)
|
bFGF
|
GRO alpha/beta/gamma
|
|
IFN-gamma
|
IGF-1
|
IL-6
|
IL-8
(CXCL8)
|
Leptin
|
|
MCP-1
(CCL2)
|
PDGF-BB
|
PLGF
|
RANTES
(CCL5)
|
TGF beta 1
|
|
TIMP-1
|
TIMP-2
|
Thrombopoietin
(TPO)
|
VEGF-A
|
VEGF-D
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Kit Components
- Human Angiogenesis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Yoon YK., Im SA., Min A., et al. Sunitinib synergizes the antitumor effect of cisplatin via modulation of ERCC1 expression in models of gastric cancer. Cancer Lett. 2012 Aug 28;321(2):128-36. doi: 10.1016/j.canlet.2012.01.019
Species:
Human
Sample Type:
Conditioned Media (Gastric Cancer Cell lines)
Ulasov I., Borovjagin A., Kaverina N., et al. MT1-MMP silencing by an shRNA-armed glioma-targeted conditionally replicative adenovirus (CRAd) improves its anti-glioma efficacy in vitro and in vivo. Cancer Lett. 2015 Sep 1;365(2):240-50. doi: 10.1016/j.canlet.2015.06.002.
Species:
Human
Sample Type:
Conditioned Media (U251 and U87 glioma cells lines)
Sze S, Kleijn D, Lai R, Tan E, Zhao H,et al.Elucidating the Secretion Proteome of Human Embryonic Stem Cell-derived Mesenchymal Stem Cells.Mol Cell Proteomics. 2007; 6: 1680-1693
Species:
Human
Sample Type:
Conditioned Media (conditioned or non-conditioned medium)
Rezzola S., Corsini M., Chiodelli P., et al. Inflammation and N-formyl peptide receptors mediate the angiogenic activity of human vitreous humour in proliferative diabetic retinopathy. Diabetologia. 2017 Apr;60(4):719-728. doi: 10.1007/s00125-016-4204-0.
Species:
Human
Sample Type:
Vitreous Fluid (Proliferative diabetic retinopathy)
Ash S., et al. Xenon decreases cell migration and secretion of a pro-angiogenesis factor in breast adenocarcinoma cells: comparison with sevoflurane. Br. J. Anaesth. (2014) doi: 10.1093/bja/aeu191
Species:
Human
Sample Type:
Conditioned Media (MDA-MB-231Human Breast adenocarcinoma Line)
Ye L., Chang Y-H., Xiong Q., et al. Cardiac Repair in a Porcine Model of Acute Myocardial Infarction with Human Induced Pluripotent Stem Cell-Derived Cardiovascular Cells. Cell Stem Cell . 2014 Dec 4;15(6):750-61. doi: 10.1016/j.stem.2014.11.009.
Species:
Human
Sample Type:
Conditioned Media (Smooth muscle cells and induced pluoripotent stem cells)
Chung E., Rytlewski J., Merchant AG., et al. Fibrin-based 3D matrices induce angiogenic behavior of adipose-derived stem cells. Acta Biomaterialia Available online 16 January 2015. doi:10.1016/j.actbio.2015.01.012
Species:
Human
Sample Type:
Conditioned Media (Adipose-derived stem celsl (ACSs))
Hossain A., Gumin J., Gao F., et al. Mesenchymal Stem Cells Isolated From Human Gliomas Increase Proliferation and Maintain Stemness of Glioma Stem Cells Through the IL-6/gp130/STAT3 Pathway. Stem Cells. 2015 Aug;33(8):2400-15. doi: 10.1002/stem.2053.
Species:
Human
Sample Type:
Conditioned Media (Mesenchymal cells in gliomas help maintain "stemness" in gioma cells)
Fierro, Fernando A., et al. "Effects on proliferation and differentiation of multipotent bone marrow stromal cells engineered to express growth factors for combined cell and gene therapy." Stem Cells 29.11 (2011): 1727-1737.
Species:
Human
Sample Type:
Conditioned Media (MSCs )
Jin YP., Hu YP., Wu XS., et al. miR-143-3p targeting of ITGA6 suppresses tumour growth and angiogenesis by downregulating PLGF expression via the PI3K/AKT pathway in gallbladder carcinoma. Cell Death Dis. 2018 Feb; 9(2): 182. 2018 Feb 7. doi: 10.1038/s41419-017-0258-2
Species:
Human
Sample Type:
Conditioned Media (Gall bladder cancner cell line, NOZ treated with microRNAs)
-
Great productI used the human angiogenesis array 1 and 2 to study the effect of some natural compound and I found a good results.
from Florida A&M unuversity,
on
Great productI used this kit to detect the angiogenesis related cytokines in plasma and it works fine.from Purdue University,
on
Write Your Own Review