TNF alpha Epitope Mapping Peptide Array
The TNF alpha Epitope Mapping Peptide Array is a semi-quantitative array displaying 222 synthesized peptides representing the full-length TNFA. It is suitable for antibody epitope mapping and detection of IgG, IgA, IgM, IgE, etc. in serum, plasma, and many other liquid sample types.
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Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit, 16 Sample Kit |
|---|---|
| Species | Human |
| Accession Number | P01375 |
| Protein Name / Synonyms | Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF) Intracellular domain 1 (ICD1) Intracellular domain 2 (ICD2) C-domain 1 C-domain 2 Tumor necrosis factor, soluble form] |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 222 |
| Compatible Sample Types | Plasma, Serum, Ascites, Hybridoma cell culture medium, Purified antibodies, Purified protein, DNA Aptamers, Other fluids |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Antibody Drug Development, Drug Antibody (DA) Detection |
| Estimated Lead Time | 4-6 weeks |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| 1. MSTESMIRDVEL | 2. STESMIRDVELA | 3. TESMIRDVELAE | 4. ESMIRDVELAEE |
| 5. SMIRDVELAEEA | 6. MIRDVELAEEAL | 7. IRDVELAEEALP | 8. RDVELAEEALPK |
| 9. DVELAEEALPKK | 10. VELAEEALPKKT | 11. ELAEEALPKKTG | 12. LAEEALPKKTGG |
| 13. AEEALPKKTGGP | 14. EEALPKKTGGPQ | 15. EALPKKTGGPQG | 16. ALPKKTGGPQGS |
| 17. LPKKTGGPQGSR | 18. PKKTGGPQGSRR | 19. KKTGGPQGSRRC | 20. KTGGPQGSRRCL |
| 21. TGGPQGSRRCLF | 22. GGPQGSRRCLFL | 23. GPQGSRRCLFLS | 24. PQGSRRCLFLSL |
| 25. QGSRRCLFLSLF | 26. GSRRCLFLSLFS | 27. SRRCLFLSLFSF | 28. RRCLFLSLFSFL |
| 29. RCLFLSLFSFLI | 30. CLFLSLFSFLIV | 31. LFLSLFSFLIVA | 32. FLSLFSFLIVAG |
| 33. LSLFSFLIVAGA | 34. SLFSFLIVAGAT | 35. LFSFLIVAGATT | 36. FSFLIVAGATTL |
| 37. SFLIVAGATTLF | 38. FLIVAGATTLFC | 39. LIVAGATTLFCL | 40. IVAGATTLFCLL |
| 41. VAGATTLFCLLH | 42. AGATTLFCLLHF | 43. GATTLFCLLHFG | 44. ATTLFCLLHFGV |
| 45. TTLFCLLHFGVI | 46. TLFCLLHFGVIG | 47. LFCLLHFGVIGP | 48. FCLLHFGVIGPQ |
| 49. CLLHFGVIGPQR | 50. LLHFGVIGPQRE | 51. LHFGVIGPQREE | 52. HFGVIGPQREEF |
| 53. FGVIGPQREEFP | 54. GVIGPQREEFPR | 55. VIGPQREEFPRD | 56. IGPQREEFPRDL |
| 57. GPQREEFPRDLS | 58. PQREEFPRDLSL | 59. QREEFPRDLSLI | 60. REEFPRDLSLIS |
| 61. EEFPRDLSLISP | 62. EFPRDLSLISPL | 63. FPRDLSLISPLA | 64. PRDLSLISPLAQ |
| 65. RDLSLISPLAQA | 66. DLSLISPLAQAV | 67. LSLISPLAQAVR | 68. SLISPLAQAVRS |
| 69. LISPLAQAVRSS | 70. ISPLAQAVRSSS | 71. SPLAQAVRSSSR | 72. PLAQAVRSSSRT |
| 73. LAQAVRSSSRTP | 74. AQAVRSSSRTPS | 75. QAVRSSSRTPSD | 76. AVRSSSRTPSDK |
| 77. VRSSSRTPSDKP | 78. RSSSRTPSDKPV | 79. SSSRTPSDKPVA | 80. SSRTPSDKPVAH |
| 81. SRTPSDKPVAHV | 82. RTPSDKPVAHVV | 83. TPSDKPVAHVVA | 84. PSDKPVAHVVAN |
| 85. SDKPVAHVVANP | 86. DKPVAHVVANPQ | 87. KPVAHVVANPQA | 88. PVAHVVANPQAE |
| 89. VAHVVANPQAEG | 90. AHVVANPQAEGQ | 91. HVVANPQAEGQL | 92. VVANPQAEGQLQ |
| 93. VANPQAEGQLQW | 94. ANPQAEGQLQWL | 95. NPQAEGQLQWLN | 96. PQAEGQLQWLNR |
| 97. QAEGQLQWLNRR | 98. AEGQLQWLNRRA | 99. EGQLQWLNRRAN | 100. GQLQWLNRRANA |
| 101. QLQWLNRRANAL | 102. LQWLNRRANALL | 103. QWLNRRANALLA | 104. WLNRRANALLAN |
| 105. LNRRANALLANG | 106. NRRANALLANGV | 107. RRANALLANGVE | 108. RANALLANGVEL |
| 109. ANALLANGVELR | 110. NALLANGVELRD | 111. ALLANGVELRDN | 112. LLANGVELRDNQ |
| 113. LANGVELRDNQL | 114. ANGVELRDNQLV | 115. NGVELRDNQLVV | 116. GVELRDNQLVVP |
| 117. VELRDNQLVVPS | 118. ELRDNQLVVPSE | 119. LRDNQLVVPSEG | 120. RDNQLVVPSEGL |
| 121. DNQLVVPSEGLY | 122. NQLVVPSEGLYL | 123. QLVVPSEGLYLI | 124. LVVPSEGLYLIY |
| 125. VVPSEGLYLIYS | 126. VPSEGLYLIYSQ | 127. PSEGLYLIYSQV | 128. SEGLYLIYSQVL |
| 129. EGLYLIYSQVLF | 130. GLYLIYSQVLFK | 131. LYLIYSQVLFKG | 132. YLIYSQVLFKGQ |
| 133. LIYSQVLFKGQG | 134. IYSQVLFKGQGC | 135. YSQVLFKGQGCP | 136. SQVLFKGQGCPS |
| 137. QVLFKGQGCPST | 138. VLFKGQGCPSTH | 139. LFKGQGCPSTHV | 140. FKGQGCPSTHVL |
| 141. KGQGCPSTHVLL | 142. GQGCPSTHVLLT | 143. QGCPSTHVLLTH | 144. GCPSTHVLLTHT |
| 145. CPSTHVLLTHTI | 146. PSTHVLLTHTIS | 147. STHVLLTHTISR | 148. THVLLTHTISRI |
| 149. HVLLTHTISRIA | 150. VLLTHTISRIAV | 151. LLTHTISRIAVS | 152. LTHTISRIAVSY |
| 153. THTISRIAVSYQ | 154. HTISRIAVSYQT | 155. TISRIAVSYQTK | 156. ISRIAVSYQTKV |
| 157. SRIAVSYQTKVN | 158. RIAVSYQTKVNL | 159. IAVSYQTKVNLL | 160. AVSYQTKVNLLS |
| 161. VSYQTKVNLLSA | 162. SYQTKVNLLSAI | 163. YQTKVNLLSAIK | 164. QTKVNLLSAIKS |
| 165. TKVNLLSAIKSP | 166. KVNLLSAIKSPC | 167. VNLLSAIKSPCQ | 168. NLLSAIKSPCQR |
| 169. LLSAIKSPCQRE | 170. LSAIKSPCQRET | 171. SAIKSPCQRETP | 172. AIKSPCQRETPE |
| 173. IKSPCQRETPEG | 174. KSPCQRETPEGA | 175. SPCQRETPEGAE | 176. PCQRETPEGAEA |
| 177. CQRETPEGAEAK | 178. QRETPEGAEAKP | 179. RETPEGAEAKPW | 180. ETPEGAEAKPWY |
| 181. TPEGAEAKPWYE | 182. PEGAEAKPWYEP | 183. EGAEAKPWYEPI | 184. GAEAKPWYEPIY |
| 185. AEAKPWYEPIYL | 186. EAKPWYEPIYLG | 187. AKPWYEPIYLGG | 188. KPWYEPIYLGGV |
| 189. PWYEPIYLGGVF | 190. WYEPIYLGGVFQ | 191. YEPIYLGGVFQL | 192. EPIYLGGVFQLE |
| 193. PIYLGGVFQLEK | 194. IYLGGVFQLEKG | 195. YLGGVFQLEKGD | 196. LGGVFQLEKGDR |
| 197. GGVFQLEKGDRL | 198. GVFQLEKGDRLS | 199. VFQLEKGDRLSA | 200. FQLEKGDRLSAE |
| 201. QLEKGDRLSAEI | 202. LEKGDRLSAEIN | 203. EKGDRLSAEINR | 204. KGDRLSAEINRP |
| 205. GDRLSAEINRPD | 206. DRLSAEINRPDY | 207. RLSAEINRPDYL | 208. LSAEINRPDYLD |
| 209. SAEINRPDYLDF | 210. AEINRPDYLDFA | 211. EINRPDYLDFAE | 212. INRPDYLDFAES |
| 213. NRPDYLDFAESG | 214. RPDYLDFAESGQ | 215. PDYLDFAESGQV | 216. DYLDFAESGQVY |
| 217. YLDFAESGQVYF | 218. LDFAESGQVYFG | 219. DFAESGQVYFGI | 220. FAESGQVYFGII |
| 221. AESGQVYFGIIA | 222. ESGQVYFGIIAL | Â | Â |
Application Notes
- Identify high antigenicity epitopes for antibody production & vaccine development
- Locate (auto)antigen epitopes of (auto)antibodies
- Define the best antibody clone against the protein-of-interest
- Perform epitope mutant analysis
- Map B cell epitopes
Service Features
- Small serum sample volume required (2 µl)
- High density (simultaneously analyze hundreds of overlapping peptides)
- High sensitivity
- Large dynamic range
- Suitable for high-throughput assays
- High efficiency and accuracy
- Affordable, quick and simple to use
Kit Components
- RayBio® human TNF alpha protein peptide array
- Blocking Buffer
- Wash Buffer I
- Wash Buffer II
- Biotin-conjugated secondary antibody
- Cy3 equivalent dye conjugated streptavidin
- Array accessories (including slide incubation chamber assembly, gasket, adhesive plastic strips, etc.)
Other Materials Required
- Distilled water
- Small plastic boxes or containers
- Orbital shaker or oscillating rocker
- Pipettors, pipette tips and other common lab consumables
- Aluminum foil
- Laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Storage/Stability
Upon receipt, all kit components should be stored at -20 °C to -80 °C until ready to use. Stored properly, the kit will retain complete activity for up to 6 months.
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