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This kit is a sandwich-based bridging ELISA for the quantitative measurement of Rituximab in serum, plasma, and cell culture supernatants.
Product Description
Specifications
Size
1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Protein Name / Synonyms
Rituximab
Quantitative/Semi-Quantitative
Quantitative
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum
Solid Support
96-well Microplate
Method Of Detection
Colorimetric
Design Principle
Sandwich-based
Research Area
Antibody Drug Development, Drug Antibody (DA) Detection
Estimated Lead Time
6-8 weeks
Shipping Type
Blue ice
Storage
≤-20°C
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Application Notes Kit Components
Pre-Coated 96-well Strip Microplate Wash Buffer Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate Stop Solution Assay Diluent Other Materials Required
Microplate reader capable of measuring absorbance at 450nm Precision pipettes to deliver 2 µl to 1 ml volumes Adjustable 1-25 ml pipettes for reagent preparation 100 ml and 1 liter graduated cylinders Absorbent paper Distilled or deionized water Log-log graph paper or computer and software for ELISA data analysis Horizontal orbital microplate shaker Tubes to prepare standard and sample dilutions Protocol Outline
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2 hours at room temperature. Add 100 μl prepared biotinylated antibody to each well. Incubate 1 hour at room temperature. Add 100 μl prepared Streptavidin solution. Incubate 45 minutes at room temperature. Add 100 μl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately. Storage/Stability The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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