Recombinant SARS-CoV S1 Subunit Protein, full length

Recombinant SARS-CoV S1 subunit protein with C-terminal His-tag, derived from the transfected human HEK293 cells.
As low as $239.00
In stock
Only %1 left
Catalog #:
230-30192
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Product Description

Specifications

Size 50 ug, 100 ug
Species SARS-CoV
Accession Number P59594
Gene Symbols S
Protein Name / Synonyms Spike protein, S Protein, S1 Subunit
Expressed Region Ser14-Arg667
Expression System HEK293 cells
Tag C-terminal his-tag
Purification His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity >95 %
Purity Determined By SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa) Recombinant protein product has a calculated molecular mass of ∼75 kDa. Due to the abundant glycosylation, it migrates as approximately 100 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Format Liquid
Formulation Supplied as a 0.2 µm filtered solution in PBS (pH 7.4)
Concentration (lot specific) Lot specific (see the label on the vial), determined by BCA protein assay
Endotoxin Level < 0.5 EU per µg of the protein as determined by the LAL method
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage -20°C
SDS-PAGE Image
SDS-PAGE
Activity
Product binding ability was measured by ELISA. The immobilized recombinant human ACE2 protein (Catalog #230-30165, coated at 0.5 µg/ml, 100 µl/well) was incubated with the serial diluted SARS CoV S1 subunit (full length) protein (Catalog #230-30192). The bound protein was detected by mouse anti-SARS CoV-2 S1 RBD monoclonal antibody (cross-reaction) using ELISA. The calculated EC50 is 126.7 ng/ml.
Binding Function

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

Custom Labeling Request

We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
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