Val16-Gln690. Y145H/A222V mutant: amino acid Tyr (Y) at 145 position was mutated to His (H), Ala (A) at 222 position was mutated to Val (V).
Expression System
HEK293 cells
Tag
C-terminal his-tag
Purification
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity
0.8
Purity Determined By
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)
Recombinant protein product has a calculated molecular mass of ∼75 kDa. Due to the abundant glycosylation, it migrates as approximately ∼120 kDa major protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Format
Liquid
Formulation
Supplied as a 0.2 µm filtered solution in PBS (pH 7.4)
Concentration (lot specific)
Lot specific (see the label on the vial), determined by BCA protein assay
Specificity
SARS-CoV-2 Delta B.1.617.2 (India)
Endotoxin Level
< 0.5 EU per µg of the protein as determined by the LAL method
Estimated Lead Time
2-3 weeks
Shipping Type
Blue ice
Storage
-20°C
SDS-PAGE Image
Figure 1. Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one reduced band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated. Lane 1: Protein standard ladder (kDa). Lane 2: Untreated protein Lane 3: Treated protein with deglycosylation enzymes under native conditions. Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Binding Function
Product binding activity to human ACE2 was confirmed by ELISA. The immobilized recombinant human ACE2 protein (Catalog #230-30165, coated at 0.5 µg/ml, 100 µl/well) was incubated with the serial diluted SARS CoV-2 S1 full length with Y145H/A222V mutant protein (Catalog #230-30211). The significant mutant protein binding was detected by mouse anti-S1 RBD monoclonal antibody.
Stability & Storage
Shipping
Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
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