Recombinant SARS-CoV-2 Spike Protein Subunit 1, N-terminal with Fc tag
Recombinant SARS-CoV-2 Spike Protein Subunit 1, N-terminal domain, with a C-terminal mouse IgG Fc tag, derived from the transfected human HEK293 cells.
Product Description
Specifications
| Size | 10 ug, 50 ug, 100 ug |
|---|---|
| Species | SARS-CoV-2 |
| Accession Number | QHD43416 |
| Gene Symbols | S |
| Protein Name / Synonyms | Spike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD) |
| Expressed Region | Val16-Phe318 |
| Expression System | HEK293 cells |
| Tag | C-terminal mouse IgG Fc tag |
| Purification | His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 70 kDa (including 25 kDa Fc-tag). Due to the abundant glycosylation, it migrates as approximately ∼80 kDa major protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below. |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by BCA protein assay |
| Endotoxin Level | <0.5 EU per ug of the protein as determined by the LAL method |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
