Recombinant SARS-CoV-2 Spike Protein, S2 Subunit
Recombinant SARS-CoV-2 Spike Protein, S2 Subunit derived from E.coli
Product Description
Specifications
| Size | 100 ug, 250 ug |
|---|---|
| Species | SARS-CoV-2 |
| Accession Number | QHD43416 |
| Protein Name / Synonyms | SARS-CoV-2 Spike Protein, S2 Subunit, S protein, spike protein |
| Expressed Region | Met697 - Pro1213 |
| Expression System | E. coli |
| Tag | N-terminal histidine tag |
| Purification | His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
| Purity | >95 % |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | ∼58 kDa |
| Format | Liquid |
| Formulation | Filtered solution in 50 mM Tris-HCl, 500 mM NaCl, 600 mM arginine, 2 mM oxidized glutathione, 4 mM reduced glutathione, 0.2% PEG4000, 20% glycerol (pH 8.5). |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by Bradford protein assay. |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
J Yu, et al. Direct activation of the alternative complement pathway by SARS-CoV-2 spike protein is blocked by factor D inhibition. Blood. 2020 Sep 2;blood.2020008248 (2020).
Prompetchara E, et al. DNA vaccine candidate encoding SARS-CoV-2 spike proteins elicited potent humoral and Th1 cell-mediated immune responses in mice. PLOS ONE 16(3): e0248007 (2021).
Bortolotti D, et al. SARS-CoV-2 spike 1 protein controls natural killer cell activation via the HLA-E/NKG2A pathway. Cells (2020).
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Recombinant SARS-CoV-2 Spike Protein, S2 Subunit derived from E.coliThe E. coli-derived spike protein that is not glycosylated exhibits some biological activities. This S2 protein has been used as a control when we assess the effects of the S1 protein.
from Georgetown University,
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