Recombinant SARS-CoV-2 Spike Glycoprotein S1 Subunit Protein, N-terminal Extracellular Domain (ECD)
Recombinant SARS-CoV-2 spike glycoprotein S1 subunit, N-terminal extracellular domain (ECD) protein with C-terminal His-tag, derived from the transfected human HEK293 cells.
Product Description
Specifications
| Size | 50 ug, 200 ug |
|---|---|
| Species | SARS-CoV-2 |
| Accession Number | QHD43416 |
| Gene Symbols | |S |
| Protein Name / Synonyms | Spike protein, S Protein, S1 Subunit |
| Expressed Region | Val16-Phe318 |
| Expression System | HEK293 cells |
| Tag | C-terminal his-tag |
| Purification | His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
| Purity | >95 % |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of ∼35 kDa. Due to the abundant glycosylation, it migrates as approximately 50-55 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one reduced ∼35 kDa band. See deglycosylation analysis image below. |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by BCA protein assay |
| Endotoxin Level | < 0.5 EU per µg of the protein as determined by the LAL method |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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SARS-CoV-2 Spike ECDThis is a nice tool to understand the mechanism of S1 protein actions. As expected, the ECD domain does not binding to ACE2.
from Georgetown University,
on
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