His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity
>90 %
Purity Determined By
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)
Recombinant protein product has a calculated molecular mass of ∼60 kDa. Due to the abundant glycosylation, it migrates as approximately 80 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below.
Format
Liquid
Formulation
Supplied as a 0.2 µm filtered solution in PBS (pH 7.4)
Concentration (lot specific)
Lot specific (see the label on the vial), determined by BCA protein assay
Estimated Lead Time
1-2 business days
Shipping Type
Blue ice
Storage
-20°C
SDS-PAGE Image
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with Protein Deglycosylation Kit under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size (˜60 kDa), thus indicating that the untreated recombinant protein (Lane 2, ˜80 kDa) was glycosylated. Lane 1: Protein standard ladder (kDa) Lane 2: Untreated protein (˜80 kDa, red arrow) under reducing conditions Lane 3: Partially cleaved protein (˜60 kDa, green arrow) with deglycosylation enzymes under native conditions Lane 4: Fully cleaved protein (˜60 kDa, green arrow) with deglycosylation enzymes under reducing conditions
Stability & Storage
Shipping
Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
Datasheet
