SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)
Recombinant protein product has a calculated molecular mass of 50 kDa including 25 kDa mouse IgG Fc-tag. Due to the abundant glycosylation, it migrates as approximately ∼65 kDa major protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below.
Format
Liquid
Formulation
Supplied as a 0.2 µm filtered solution in PBS (pH 7.4)
Concentration (lot specific)
Lot specific (see the label on the vial), determined by BCA protein assay
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with deglycosylation under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size (˜50 kDa), thus indicating that the untreated recombinant protein (Lane 2, ˜65 kDa) was glycosylated. Lane 1: Protein standard ladder (kDa) Lane 2: Untreated protein (˜65 kDa, red arrow) under reducing conditions. Lane 3: Treated protein (˜50 kDa, green arrow) with deglycosylation enzymes under native conditions. Lane 4: Treated protein (˜50 kDa, green arrow) with deglycosylation enzymes under reducing conditions.
Activity
Measured by its binding ability in a functional ELISA. Recombinant S1/RBD-mFc (Code:Â 230-30166) can bind immobilized recombinant human ACE2 (Code:Â 230-30165) with EC50Â at 3.2 ng/mL.
Stability & Storage
Shipping
Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
Datasheet
