Recombinant SARS-CoV-2 S1 Subunit Protein RBD, R346K/E484K/N501Y mutant, Biotinylated
Biotin-conjugated recombinant SARS-CoV-2 S1 subunit protein RBD, mutant R346K/E484K/N501Y with C-terminal His-tag, derived from the transfected human HEK293 cells.
Product Description
Specifications
| Size | 100 ug, 500 ug, 1000 ug |
|---|---|
| Species | SARS-CoV-2 |
| Accession Number | QHD43416 |
| Gene Symbols | S |
| Protein Name / Synonyms | Spike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD) |
| Expressed Region | Arg319-Phe541. R346K/E484K/N501Y mutant: amino acid Arg (R) at 346 position was mutated to Lys (K), Glu (E) at 484 position was mutated to Lys (K), Asn (N) at 501 position was mutated to Tyr (Y). |
| Expression System | HEK293 cells |
| Conjugation | Biotin |
| Tag | C-terminal his-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
| Purification | His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of ~25 kDa. Due to the abundant glycosylation, it migrates as approximately ~30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one reduced ~25 kDa band. |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by BCA protein assay |
| Specificity | SARS-CoV-2 Mu B.1.621 (Colombia) |
| Endotoxin Level | < 0.5 EU per µg of the protein as determined by the LAL method |
| Protein Type | Recombinant Protein |
| Estimated Lead Time | 2-3 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
