Recombinant SARS CoV-2 Omicron XBB.1 S1RBD Protein

Recombinant SARS CoV-2 Omicron XBB.1 S1RBD with a C-terminal His-tag, derived from the transfected human HEK293 cells.
As low as $119.00
In stock
Only %1 left
Catalog #:
230-30247
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Product Description

Specifications

Size 10 ug
Species SARS-CoV-2
Accession Number QHD43416
Gene Symbols S
Protein Name / Synonyms SARS-CoV-2 Spike Protein, S1 Subunit, S protein, spike protein
Expressed Region Arg319-Phe541. 22 site mutation: G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486S, F490S, Q498R, N501Y, Y505H.
Expression System HEK293 cells
Tag C-terminal histidine tag
Purification His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity >95%
Purity Determined By SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa) Recombinant protein product has a calculated molecular mass of 25 kDa. Due to the abundant glycosylation, it migrates as approximately 35 kDa major protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below.
Format Liquid
Formulation Supplied as a 0.2 µm filtered solution in PBS (pH 7.4)
Concentration (lot specific) Lot specific (see the label on the vial), determined by Bradford protein assay
Endotoxin Level <0.5 EU per ugof the protein as determined by the LAL method
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage -20°C
Storage / Stability Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
SDS-PAGE Image
Figure 1. Deglycosylation analysis of purified recombinant proteins. Lane 1, protein ladder (kDa). Lane 2, glycosylated protein (delivered to customers). Lanes 3 and 4, deglycosylated proteins under native and denature conditions respectively.

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