Recombinant SARS-CoV-2 Omicron Variant BA.2 Spike S1 RBD Protein with 16 Mutant Sites
Recombinant SARS-CoV-2 (2019-nCoV) Omicron variant spike S1 subunit receptor binding protein (RBD) with 16 mutant sites (G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H) with C-terminal His-tag, derived from the transfected human HEK293 cells.
Product Description
Specifications
| Size | 10 ug |
|---|---|
| Species | SARS-CoV-2 |
| Accession Number | QHD43416 |
| Gene Symbols | S |
| Protein Name / Synonyms | Spike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD) |
| Expressed Region | Arg319-Phe541, 16 mutant sites (G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H). |
| Expression System | HEK293 cells |
| Tag | C-terminal his-tag |
| Purification | His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
| Purity | >80% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 25 kDa. Due to the abundant glycosylation, it migrates as approximately ∼30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one reduced ∼25 kDa band. |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by BCA protein assay |
| Specificity | SARS-CoV-2 Omicron Variant BA.2 |
| Endotoxin Level | < 0.5 EU per ug of the protein as determined by the LAL method |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
