Recombinant SARS-CoV-2 Omicron Variant BA.2.75.2 Spike S1 RBD Protein
Recombinant SARS-CoV-2 Omicron variant BA.2.75.2 spike S1 subunit receptor binding protein (RBD) with 19 mutant sites (G339H, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, G446S, N460K, S477N, T478K, E484A, F486S, Q498R, N501Y, Y505H) with C-terminal His-tag, derived from the transfected human HEK293 cells.
Product Description
Specifications
| Size | 10 ug |
|---|---|
| Species | SARS-CoV-2 |
| Accession Number | QHD43416 |
| Gene Symbols | S |
| Protein Name / Synonyms | Spike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD) |
| Expressed Region | Arg319-Phe541, 19 mutant sites (G339H, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, G446S, N460K, S477N, T478K, E484A, F486S, Q498R, N501Y, Y505H). |
| Expression System | HEK293 cells |
| Tag | C-terminal his-tag |
| Purification | His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 25 kDa. Due to the abundant glycosylation, it migrates as approximately 30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by Bio-Rad protein assay reagent |
| Specificity | SARS-CoV-2 Omicron Variant BA.2.75.2 |
| Endotoxin Level | <0.5 EU per ug of the protein as determined by the LAL method |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Stability & Storage
Shipping
Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.