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Recombinant SARS-CoV-2 Omicron Variant B.1.1.529 Nucleocapsid (N) Protein
Recombinant SARS-CoV-2 Omicron Variant B.1.1.529 Nucleocapsid (N), derived from the transfected human HEK293 cells.
Product Description
Specifications
| Size | 50 ug, 100 ug |
|---|---|
| Species | SARS-CoV-2 |
| Accession Number | QHD43423 |
| Gene Symbols | N |
| Protein Name / Synonyms | Nucleocapsid Protein, N Protein |
| Expressed Region | Met1-Ala419, containing 4 deletions (P13L, E31del, R32del, and S33del) and 2 mutations (R203K, G204R). |
| Expression System | HEK293 cells |
| Tag | C-terminal His-tag |
| Purification | His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 47 kDa. Due to the abundant glycosylation, it migrates as approximately 55 kDa major protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. The minor small protein bands (25-30 kDa) likely the cleaved products. See deglycosylation analysis image below. |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by BCA protein assay |
| Specificity | SARS-CoV-2 Omicron Variant B.1.1.529 |
| Endotoxin Level | <0.5 EU per ug of the protein as determined by the LAL method |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Lane 1: protein standard ladder (kDa).
Lane 2: untreated protein.
Lane 3: treated protein with deglycosylation enzymes under native conditions.
Lane 4: treated protein with deglycosylation enzymes under denature conditions.
Stability & Storage
Shipping
Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.