Recombinant Mouse ACE2 Protein

Recombinant mouse angiotensin-converting enzyme 2 (ACE2) with C-terminal his-tag, derived from the transfected human HEK293 cells.
As low as $313.00
In stock
Only %1 left
Catalog #:
230-30177
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Product Description

Specifications

Size 100 ug, 500 ug, 1000 ug
Species Mouse
Accession Number Q8R0I0
Gene Symbols ACE2
Protein Name / Synonyms ACE2, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH, Metalloprotease MPROT15
Expressed Region Gln18-Thr740 (Extracellular domain)
Expression System HEK293 cells
Tag C-terminal his-tag
Purification His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity >95%
Purity Determined By SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa) Recombinant protein product has a calculated molecular mass of ∼80 kDa. Due to the abundant glycosylation, it migrates as approximately ∼90 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one single ∼80 kDa band. See deglycosylation analysis image below.
Format Liquid
Formulation Supplied as a 0.2 µm filtered solution in PBS (pH 7.4)
Concentration (lot specific) Lot specific (see the label on the vial), determined by BCA protein assay
Endotoxin Level < 0.5 EU per µg of the protein as determined by the LAL method
Research Area Angiogenesis
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage -20°C
SDS-PAGE Image
SDS-PAGE
Figure 1.Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one reduced band at the expected size (˜80 kDa), thus indicating that the untreated recombinant protein (Lane 2, ˜90 kDa) was glycosylated.
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein under reducing conditions.
Lane 3: Treated protein with deglycosylation enzymes under native conditions.
Lane 4: Treated protein with deglycosylation enzymes under denature conditions.
Lane 5: Deglycosylation mixture only without target proteins.

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

Custom Labeling Request

We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
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