Recombinant Mpox Virus IMV Surface Membrane 14-kDa Fusion Protein, A29L

Recombinant Mpox (formerly known as monkeypox) virus IMV surface membrane 14-kDa fusion protein (A29L) with a C-terminal His-tag, derived from the transfected HEK293 cells.
$313.00
In stock
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Product Description

Specifications

Size 100 ug
Species Mpox Virus (MPXV)
Accession Number Q90188
Gene Symbols A29L
Expressed Region Met1-Glu110
Expression System HEK293 cells
Tag C-terminal His-tag
Purification His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity >95%
Purity Determined By SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa) Recombinant protein product has a calculated molecular mass of ∼13 kDa. Due to the abundant glycosylation, it migrates as two major bands: approximately ∼13 kDa non-glycosylated form and ∼16 kDa glycosylated form in SDS-PAGE under DTT and beta-mercaptoethanol reducing conditions.
Format Liquid
Formulation Filtered solution in PBS with 1% mannitol and 5% trehalose
Concentration (lot specific) Bio-Rad protein assay reagent
Endotoxin Level <0.5 EU per µg of the protein as determined by the LAL method
Research Area Mpox
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage -20°C
SDS-PAGE
SDS-PAGE
Figure 1. Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one reduced band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.
Lane 1: protein standard ladder (kDa).
Lane 2: untreated protein.
Lane 3: treated protein with deglycosylation enzymes under native conditions.
Lane 4: treated protein with deglycosylation enzymes under denature conditions.

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
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