Recombinant Mpox Virus Cell Surface-binding Protein, E8L
Recombinant Mpox (formerly known as monkeypox) virus (MPXV) cell surface-binding protein E8L with C-terminal His-tag, derived from the transfected human HEK293 cells.
Product Description
Specifications
| Size | 100 ug, 250 ug |
|---|---|
| Species | Mpox Virus (MPXV) |
| Accession Number | Q8V4Y0 |
| Gene Symbols | E8L |
| Protein Name / Synonyms | Cell surface-binding protein, carbonic anhydrase homolog, E8L |
| Expressed Region | Met1-Thr275 |
| Expression System | HEK293 cells |
| Tag | C-terminal his-tag |
| Purification | His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of ∼32 kDa. Due to the abundant glycosylation, it migrates as approximately ∼40 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4) with 1% mannitol and 5% trehalose |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water. |
| Endotoxin Level | <0.5 EU per ug of the protein as determined by the LAL method |
| Research Area | Mpox |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
