Recombinant Human TNF alpha Protein

Recombinant human tumor necrosis factor /TNF alpha protein with a C-terminal His-tag, derived from the transfected human HEK293 cells.
$239.00
In stock
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Product Description

Specifications

Size 50 µg
Species Human
Accession Number P01375
Gene Symbols TNF, TNFA
Protein Name / Synonyms Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2 (TNF-a).
Expressed Region Val77-Leu233
Expression System HEK293 cells
Tag C-terminal His-tag
Purity >95%
Purity Determined By SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa) Recombinant protein product has a calculated molecular mass of 17 kDa.
Format Liquid
Formulation Supplied as a 0.2 µm filtered solution in 1x PBS (pH 7.4) with 1% mannitol and 5% trehalose
Concentration (lot specific) Refer to the label on the vial for the specific concentration, determined by the Pierce BCA Protein Assay Kit.
Endotoxin Level < 0.5 EU per μg of the protein as determined by the LAL method
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage -20°C
SDS-PAGE Image
Activity
Decreasing cell viability significantly. Measured in a cytotoxicity assay using MCF‑7 human breast cancer cells. The EC50 for this effect is 4.7 ng/mL.
Storage/Stability
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Upon arrival, the liquid protein may be stored for 2 weeks at 4 °C. For long-term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Generally, the shelf life is up to 6 months from the date of receipt at -20 °C or -80 °C under sterile condition.
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