Recombinant Human Neuropilin-1 (NRP1) Protein, Isoform 1, Triple Mutant (S346A, E348A, T349A), Biotinylated
Biotin-conjugated recombinant human neuropilin-1 (NRP1, isoform 1), triple mutant (S346A, E348A, T349A) with C-terminal His-tag, derived from the transfected human HEK293 cells.
Product Description
Specifications
| Size | 100 ug, 50 ug, 500 ug |
|---|---|
| Species | Human |
| Accession Number | O14786 (isoform 1) |
| Gene Symbols | NRP1 |
| Protein Name / Synonyms | Neuropilin-1, vascular endothelial cell growth factor 165 receptor, NRP-1, CD304 |
| Expressed Region | Phe22-Pro856. Three amino acid residue at Ser346, Glu348 and Thr349 were mutated to Ala (A) and generated NRP1 triple mutant (S346A, E348A, and T349A). |
| Expression System | HEK293 cells |
| Conjugation | Biotin |
| Tag | C-terminal his-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
| Purification | His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of ∼95 kDa. Due to the abundant glycosylation, it migrates as approximately ∼120 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one reduced ∼110 kDa band. See deglycosylation analysis image below. |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by BCA protein assay |
| Endotoxin Level | < 0.5 EU per µg of the protein as determined by the LAL method |
| Research Area | Neuroscience |
| Protein Type | Recombinant Protein |
| Estimated Lead Time | 2-3 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
