Recombinant Human IGFBP-3 Protein, HEK293
Recombinant human IGFBP-3 protein, derived from the transfected human HEK293 cells.
Product Description
Specifications
| Size | 10 ug, 50 ug |
|---|---|
| Species | Human |
| Accession Number | P17936 |
| Gene Symbols | IGFBP3 |
| Protein Name / Synonyms | Insulin-Like Growth Factor Binding Protein 3, IGF-Binding Protein 3, Acid Stable Subunit Of The 140 K IGF Complex, Growth Hormone-Dependent Binding Protein, Binding Protein 53, Binding Protein 29, BP-53 |
| Expressed Region | Gly28-Lys291 |
| Expression System | HEK293 cells |
| Tag | N-terminal His-tag |
| Purification | His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 31 kDa. Due to the abundant glycosylation, it migrates as approximately 40 kDa major protein band and 30 kDa and 16 kDa minor bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. The small 16 kDa band is likely the cleaved products. See deglycosylation analysis image below. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Determined by BCA protein assay |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Endotoxin Level | <0.5 EU per ug of the protein as determined by the LAL method |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
