Recombinant Human Coagulation Factor II (F2) Protein, Biotinylated
Biotin-conjugated recombinant human coagulation factor II protein (F2), derived from the transfected human HEK293 cells.
Product Description
Specifications
| Size | 10 ug, 50 ug, 100 ug, 200 ug |
|---|---|
| Species | Human |
| Accession Number | P00734 |
| Gene Symbols | F2 |
| Protein Name / Synonyms | Coagulation Factor II |
| Expressed Region | Ala44-Glu622 |
| Expression System | HEK293 cells |
| Conjugation | Biotin |
| Tag | N-terminal his-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
| Purification | His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 65 kDa. Due to the abundant glycosylation, it migrates as approximately 75 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under denature condition, the protein presented as one single 65 kDa band. See deglycosylation analysis in SDS-PAGE image. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Concentration (lot specific) | Determined by BCA protein assay |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Protein Type | Recombinant Protein |
| Estimated Lead Time | 2-3 business days |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
