Recombinant Human CKM Protein

Recombinant human creatine kinase M-type/CKM protein with a C-terminal His-tag, derived from the transfected human HEK293 cells.
$239.00
In stock
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Product Description

Specifications

Size 50 µg
Species Human
Accession Number P06732
Gene Symbols CKM
Protein Name / Synonyms Creatine Kinase, M-Type, Creatine Phosphokinase M-Type, Creatine Kinase, Muscle.
Expressed Region Pro2-Asn381
Expression System HEK293 cells
Tag C-terminal His-tag
Purity >90%
Purity Determined By SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa) Recombinant protein product has a calculated molecular mass of 42 kDa. Due to the abundant glycosylation, it migrates as approximately 50 kDa major protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Format Liquid
Formulation Supplied as a 0.2 µm filtered solution in 1x PBS (pH 7.4) with 1% mannitol and 5% trehalose
Concentration (lot specific) Refer to the label on the vial for the specific concentration, determined by the Pierce BCA Protein Assay Kit.
Endotoxin Level < 0.5 EU per μg of the protein as determined by the LAL method
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage -20°C
SDS-PAGE Image
Figure 1. Deglycosylation analysis of purified recombinant proteins. Product was either untreated (Lane 2) or treated with deglycosylation enzymes under native (Lane 3) or denaturing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift, indicating that the untreated protein (Lane 2) was glycosylated. Lane 1, protein ladder (kDa).
Storage/Stability
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Upon arrival, the liquid protein may be stored for 2 weeks at 4 °C. For long-term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Generally, the shelf life is up to 6 months from the date of receipt at -20 °C or -80 °C under sterile condition.
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