Recombinant Human Serpin F2 (Alpha-2-Antiplasmin) Protein

Recombinant human alpha-2-antiplasmin (Serpin F2), derived from the transfected human HEK293 cells.
As low as $94.00
In stock
Only %1 left
Catalog #:
230-30171
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Product Description

Specifications

Size 10 ug, 50 ug, 100 ug, 200 ug
Species Human
Accession Number P08697
Gene Symbols SERPINF2
Protein Name / Synonyms Serpin Family F Member 2, Alpha-2-Plasmin Inhibitor, Serine (Or Cysteine) Proteinase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 2, Serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 2, ALPHA-2-PI, Alpha-2-AP, Serpin F2, PLI, AAP, A2AP, API
Expressed Region Asn40-Lys491
Expression System HEK293 cells
Tag N-terminal his-tag
Purification His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity >95%
Purity Determined By SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa) Recombinant protein product has a calculated molecular mass of 50 kDa. Due to the abundant glycosylation, it migrates as approximately 60 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under denature condition, the protein presented as one single 50 kDa band. See deglycosylation analysis in SDS-PAGE image.
Format Lyophilized powder
Formulation Lyophilized from a 0.2 µm filtered solution in PBS
Concentration (lot specific) Determined by BCA protein assay
Reconstitution Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
Estimated Lead Time 1-2 business days
Shipping Type Ambient temperature
Storage -20°C
SDS-PAGE Image
SDS-PAGE
Figure 1. Deglycosylation of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein under reducing conditions.
Lane 3: Treated protein with deglycosylation enzymes under native conditions.
Lane 4: Treated protein with deglycosylation enzymes under denature conditions.
Lane 5: Deglycosylation mixture only without target proteins.

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C.  For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.

Custom Labeling Request

We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
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