Recombinant HCov-HKU1 S1 subunit, RBD Protein
Recombinant HCov-HKU1 S1 subunit RBD with C-terminal His-tag, derived from the transfected human HEK293 cells.
Product Description
Specifications
| Size | 10 ug, 50 ug |
|---|---|
| Species | HCov-HKU1 |
| Accession Number | Q5MQD0 |
| Gene Symbols | S |
| Expressed Region | Thr310-Tyr624 |
| Expression System | HEK293 cells |
| Tag | C-terminal his-tag |
| Purification | His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of ∼35 kDa. Due to the abundant glycosylation, it migrates as approximately ∼60 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under denature conditions, the protein presented as one reduced ∼35 kDa band. See deglycosylation analysis image below. |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by BCA protein assay |
| Endotoxin Level | < 0.5 EU per µg of the protein as determined by the LAL method |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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HKU1HKU-1 S1 RBD can serve as a nice negative control to study the actions of SARS-CoV-2 S1 RBD.
from Georgetown University,
on
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