This RayPlex® kit is a cytometric bead array (CBA) to quantitatively measure, by flow cytometry, 12 mouse chemokines. The assay has been optimized for serum and cell culture supernatant.
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
RayPlex™ Multiplex Bead Cocktail
1X Assay Diluent
20X Wash Buffer
RayBio® Serum Diluent
Lyophilized Protein Standard Mix
1X Biotinylated Detection Antibody Cocktail
10X Streptavidin-PE
Flow Cytometer Setup Bead Cocktail
V-bottom 96-well Microplate
Filter 96-well Microplate
Other Materials Required
Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
1.5 ml polypropylene microcentrifuge tubes or similar
Microcentrifuge
If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
Optional: High throughput sampler (HTS) for 96-well plate reading
Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!
Protocol Outline
Prepare Reagents, Standards, and Samples
Add RayPlex™ Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis
Storage/Stability
Upon receipt, all components should be stored at 4°C. The Lyophilized Protein Standard Mix (Item 5) should be stored at -20°C or below. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.
