Soluble proteins play vital roles in homeostasis and disease development, including innate immunity, apoptosis, angiogenesis, cell growth, and differentiation. Thus, protein profiling has research and clinical applications, such as disease biomarker discovery and drug development. The traditional technique for detecting and quantifying soluble proteins is the enzyme-linked immunosorbent assay (ELISA), which only allows detection of one analyte each time. However, using this method to detect multiple proteins – particularly for precious samples with small quantities – becomes challenging as the processing time and required sample volume increase.
RayPlex® is a multiplexed sandwich- and bead-based quantitative antibody array for the simultaneous detection of multiple proteins using small sample volumes. It couples the versatility of RayBiotech's vast antibody pair library with familiar, reliable flow cytometry methodology. Together this creates a multiplex bead-based array requiring no dedicated instrumentation, only common flow cytometers equipped with blue (or green) and red lasers.
The RayPlex® Human Cytokine-175 Magnetic Bead Array Kit is designed to assay 175 common human cytokines simultaneously. The kit uses magnetic beads that allow the use of an automatic plate washing system with magnetic separation ability during washing steps. For data in this manual, a BioTek 405 TS Washer and LifeSep™ 96F magnetic separator unit were used. Further details are included in Section VI.
Eotaxin-2 / MPIF-2 / CCL24, BLC / BCA-1 / CXCL13, RANTES / CCL5, IL-10 / IL10, MCP-1 / CCL2, PDGF-BB / PDGFB, IFN-gamma / IFNG, TNF alpha / TNFA, IL-5 / IL5, IL-1 beta / IL-1F2 / IL1B, IL-6 / IL6, IL-2 / IL2, IL-4 / IL4, GCSF / CSF3, IL-12 p70 / IL12A, IL-8 / IL8 / CXCL8, MIP-1 alpha / CCL3, IL-7 / IL7, IL-9 / IL9, TIMP-2 / TIMP2, TNF R1 / TNFRSF1A, IL-1Ra / IL-1RN / IL-1 F3 / IRAP / IL1RN, TNF R2 / TNFRSF1B, IL-17A / IL17A, I-309 / TCA-3 / CCL1, IL-12 p40 / IL12B, IL-13 / IL13, IL-15 / IL15, Eotaxin / CCL11, IL-16 / CXCR4 / IL16, MIP-1 delta / CCL15, TNF beta / TNFSF1 / LT-alpha / TNFB, IL-1 alpha / IL-1F1 / IL1A, ICAM-1 / CD54 / ICAM1, IL-11 / IL11, IFN-alpha / IFNA1, M-CSF / CSF1, Common beta Chain / GM-CSF R beta / CSF2RB / GM-CSF, IL-28A / IFN lambda 2 / IL28A, MIP-3 beta / CCL19, MIG / CXCL9, TSLP, Eotaxin-3 / CCL26, MIP-1 beta / CCL4, MDC / CCL22, I-TAC / CXCL11, TECK / CCL25, CCL16 / HCC-4, IL-18BPa / IL18BP, NAP-2 / TCK-1 / CXCL7 / PPBP, Osteopontin / SPP1, MIF, LIF, Axl / UFO, Betacellulin / BTC, ENA-78 / CXCL5 / LIX, Lymphotactin / XCL1, SDF-1 alpha / CXCL12 alpha, CK beta 8-1 / CCL23 / MPIF-1, CXCL1 / GRO alpha / KC / CINC-1, PARC / CCL18, IP-10 / CXCL10, MIP-3 alpha / CCL20, TARC / CCL17 / ABCD-2, MCP-2 / CCL8, MCP-3 / MARC / CCL7, GCP-2 / CXCL6, CCL14 / HCC-1 / HCC-3, IL-31 / IL-31R alpha / IL31RA, IL-29 / IL29 / IFN-lambda 1 / IL-10R beta / IL10RB, IL-17F / IL17F, MCP-4 / CCL13, MEC / CCL28, CTACK / CCL27, AgRP, Angiostatin / Plasminogen / PLG, LAP / TGF beta 1 / TGFB1, IL-2R alpha / IL2RA, Angiogenin / ANG, PDGF-AB, E-Cadherin / CDH1, Activin A / INHBA, Cripto-1 / TDGF1, ICAM-2 / CD102 / ICAM2, IL-2R beta / CD122 / IL2RB, VEGFR1 / FLT1, FLRG / Follistatin / FST, Cathepsin S / CTSS, CD40 / TNFRSF5, Fc gamma RIIB/C / CD32B/C / FCGR2B/C, gp130 / sgp130 / IL6ST, Thrombopoietin / TPO / THPO, DAN / PARN, Galectin-7 / LGALS7, Fas Ligand / TNFSF6 / CD178 / FASLG, SDF-1 beta / CXCL12 beta, VEGFC, TGF beta 2 / TGFB2, IL-13R alpha 1 / IL13RA1, IL-1R-4 / ST2 / IL-33R / IL1RL1, Tie-2 / TEK / TIE2, TSH / TSHB, Sonic Hedgehog N-Terminal / Shh-N, TREM-1 / TREM1, IL-23 / IL23A / IL-23 p19, TRAIL R4 / TNFRSF10D, Alpha-Fetoprotein / AFP, Angiopoietin-like protein 4 / ANGPTL4, CA125 / MUC16, FSH / FSHB, hCG beta / CGB3, IGF-1 R / IGF1R, IL-1R-2 / IL-1RT-2 / IL-1R beta / CD121b / IL1R2, IL-18R beta / IL-1R-7 / AcPL / IL-18RAcP / IL18RAP, IL-21 / IL21, IL-3 / IL3, Leptin / Lenep, MMP-10 / MMP10, MMP-13 / MMP13 / Pro-MMP-13, MMP-3 / MMP3, MMP-8 / MMP8, Enolase 2 / ENO2 / NSE, Oncostatin M / OSM, Prolactin / PRL, PSA-free / KLK3, Siglec-9 / CD329 / SIGLEC9, ADAM-17 / TACE / ADAM17, TIMP-4 / TIMP4, TRAIL R3 / TNFRSF10C, DR6 / TNFRSF21, CD14, CEACAM1, IL-1R-1 / IL-1RT-1 / IL-1R alpha / CD121a / p80 / IL1R1, PECAM-1 / CD31 / PECAM1, E-Selectin, Endoglin / CD105 / ENG, Trappin-2 / PI3, ErbB3, CD30 / TNFRSF8, IL-2R gamma / Common gamma Chain / IL2RG, CD80 / B7-1, Contactin-2 / TAG1 / CNTN2, uPAR / PLAUR, Dtk / TYRO3, ALCAM / CD166, LIMPII / LIMP2 / SCARB2, MICA, TIM-1 / KIM-1 / HAVCR1, IL-17RA / IL17RA, XEDAR / EDA2R, Fas / Apo-1 antigen / TNFRSF6 / CD95, IL-21R / IL21R, CD40 Ligand / TNFSF5 / CD40LG, Flt-3 Ligand / FLT3L, GITR / TNFRSF18, GDF-15 / GDF15, Osteoprotegerin / TNFRSF11B / OPG, VEGFD / FIGF, beta-NGF, Growth Hormone / GH1, IGF-1 / IGF1, NGFR / TNFRSF16, TGF beta 3 / TGFB3, BMP-4 / BMP4, IGFBP-2 / IGFBP2, BMP-7 / BMP7, VEGFA, bFGF / FGF-2 / FGF2, LAP / TGF beta 1 / TGFB1, SCF / KITLG, IGFBP-6 / IGFBP6, VEGFR3 / FLT4, KDR / VEGFR2, PIGF, EG-VEGF / PK1 / PROK1
Upon receipt, the Lyophilized Protein Standard Mix should be stored at -20°C or below. Other components should be stored at 4°C. Use reagents immediately after preparation. Additional vials of the Lyophilized Protein Standard Mix can be purchased separately. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.