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    Anti-SARS-CoV-2 S1 RBD Antibody

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    2 Citations
    Polyclonal Rabbit antibody against SARS-CoV-2 (COVID-19) Spike Protein S1 Receptor Binding Domain
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    As low as $115.00
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    Catalog #:
    130-10759
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    Product Description

    Specifications

    Size 20 µg, 100 µg, 200 µg, 500 µg, 1000 µg
    Host Species Rabbit
    Accession Number QHD43416
    Protein Name / Synonyms SARS-CoV-2 S1RBD
    Clonality Polyclonal
    Preparation This antibody was produced from antiserum of rabbits immunized with a recombinant SARS-CoV-2 S1 RBD protein. The IgG fraction was purified by Protein A affinity chromatography.
    Recommended Applications ELISA (recommended work dilution = 1:160,000)
    Reconstitution Product is supplied as a powder obtained from lyophilization of purified antibody in 1X PBS, aliquot using ddH2O. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml.
    Estimated Lead Time 1-2 business days
    Shipping Type Ambient temperature
    Storage -20°C

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    Description

    It's been reported that 2019-nCoV (COVID-19) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

    Specificity

    This antibody was selected for its ability to bind to the virus SARS-CoV-2 Spike Protein Receptor Binding Domain (RBD) from the disease COVID-19.

    Storage

    Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for at least 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.

    Expiration

    12 months from the date of shipment when stored properly.

    Citations

    McCafferty S, Haque AA, Vandierendonck A, Weidensee B, Plovyt M, Stuchlíková M, François N, Valembois S, Heyndrickx L, Michiels J, Ariën KK. A dual-antigen self-amplifying RNA SARS-CoV-2 vaccine induces potent humoral and cellular immune responses and protects against SARS-CoV-2 variants through T cell-mediated immunity. Molecular Therapy. 2022 Sep 7;30(9):2968-83.
    View Publication
    Azad T, Singaravelu R, Taha Z, Jamieson TR, Boulton S, Crupi MJ, Martin NT, Fekete EE, Poutou J, Ghahremani M, Pelin A. Nanoluciferase complementation-based bioreporter reveals the importance of N-linked glycosylation of SARS-CoV-2 S for viral entry. Molecular Therapy. 2021 Jun 2;29(6):1984-2000.
    View Publication

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