Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4, BAX, BCL2L4
Clonality
Polyclonal
Clone
RB50120
Isotype
kappa, Rabbit
Recommended Applications
Western Blotting
Research Area
Apoptosis
Estimated Lead Time
1-2 weeks
Shipping Type
Blue ice
Storage
-20°C
Introduction
Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Other Information
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Calculated Molecular Weight (Da)
21184
Antigen Source
Human
Images
WB (1:2000)
Anti-BAX Antibody (N-term) at 1:2000 dilution + U-2OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.