Porcine Cytokine Array Q50
Quantibody® Porcine Cytokine Antibody Array 50 Kit. A combination of 5 non-overlapping arrays to quantitatively measure 50 Porcine cytokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
|---|---|
| Species | Porcine (pig) |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 50 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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$5 Amazon gift card in every kit box purchased.
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
|
Angiopoietin-1
|
CCL3L1
|
Decorin
|
Eotaxin-1
(CCL11)
|
Erythropoietin
|
|
GASP-1
(WFIKKNRP)
|
GM-CSF
|
IFN alpha
|
||
|
IFN-beta
|
IFN-gamma
|
IGF-2
|
IL-1 alpha
(IL-1 F1)
|
|
|
IL-1 beta
(IL-1 F2)
|
IL-1 Ra
(IL-1 F3)
|
IL-10
|
IL-12 p70
|
IL-13
|
|
IL-15
|
IL-17A
|
IL-17F
|
IL-18
|
|
|
IL-22
|
IL-4
|
IL-6
|
IL-8
(CXCL8)
|
|
|
Insulin
|
IP-10
(CXCL10)
|
MCP-1
(CCL2)
|
MIF
|
MIG
(CXCL9)
|
|
MIP-1 beta
(CCL4)
|
NCAM-1
(CD56)
|
Osteoprotegerin
(TNFRSF11B)
|
PDGF-BB
|
PECAM-1
(CD31)
|
|
RANTES
(CCL5)
|
TGF alpha
|
|||
|
TIMP-1
|
TIMP-2
|
TNF alpha
|
TWEAK R
(TNFRSF12)
|
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Porcine Cytokine Array Q50 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- 30 ml Centrifuge Tube
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
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Great productWe used the kit for porcine heart samples and it turned out to be very impressive. The overall protocol is very simple and straightforward. Will buy more assay kits from Raybiotech!
from UNC chapel hill,
on
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