Quantibody® Porcine (pig) Cytokine Array 1 Kit. Detects 10 Porcine (pig) Cytokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
|---|---|
| Species | Porcine (pig) |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 10 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
GM-CSF
| IFN-gamma
| IL-1 beta (IL-1 F2)
| IL-10
| IL-12 p70
|
IL-4
| IL-6
| IL-8 (CXCL8)
| TGF beta 1
| TNF alpha
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Porcine Cytokine Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Sohn EH., Jiao C., Kaalberg E., et al. Allogenic iPSC-derived RPE cell transplants induce immune response in pigs: a pilot study. Sci Rep. 2015 Jul 3;5:11791. doi: 10.1038/srep11791.
Species:
Porcine
Sample Type:
Vitreous Fluid (Vitreous Fluid)
Pazos, M. R., Mohammed, N., Lafuente, H., Santos, M., MartÃÂnez-Pinilla, E., Moreno, E., ... & Hillard, C. J. (2013). Mechanisms of cannabidiol neuroprotection in hypoxic-ischemic newborn pigs: Role of 5HT 1A and CB2 receptors. Neuropharmacology, 71, 282-291.
Species:
Porcine
Sample Type:
Tissue Lysate (brain)
Mulloy DP, Stone ML, Crosby IK, et al. Ex Vivo Rehabilitation of Non-Heart-Beating Donor Lungs in a Preclinical Porcine Model: Delayed Perfusion Results in Superior Lung Function. The Journal of thoracic and cardiovascular surgery. 2012;144(5):1208-1216. doi:10.1016/j.jtcvs.2012.07.056.
Species:
Porcine
Sample Type:
BAL Fluid (Porcine lungs treated with different levels of cold static presevation)
Goetz J., Fredericks D., Petersen E., et al. A clinically realistic large animal model of intra-articular fracture that progresses to post-traumatic osteoarthritis. Osteoarthritis Cartilage. 2015 Oct;23(10):1797-805. doi: 10.1016/j.joca.2015.05.022
Species:
Porcine
Sample Type:
Synovial Fluid (Synovial fluid from post-traumatic osteoarthritis model)
Butz D., et al. The expired breath carbon delta value is a marker for the onset of sepsis in a swine model. Journal of Analytical Atomic Spectrometry. 2014, 29, 606-613. DOI: 10.1039/C3JA50340B
Species:
Porcine
Sample Type:
Plasma
Beltran-Beck B., de la Fuente J., Garrido J., Aranaz A., Sevilla I., et al. Oral Vaccination with Heat Inactivated Mycobacterium bovis Activates the Complement System to Protect against Tuberculosis. PLOS One, 2014 May 19, DOI: 10.1371/journal.pone.0098048
Species:
Porcine
Sample Type:
Serum (Wild Boar Serum from vacinated and unvaccinated animals)
Beltran-Beck B., Romero B., Boadella M., et al. Tonsils of the Soft Palate Do Not Mediate the Response of Pigs to Oral Vaccination with Heat-Inactivated Mycobacterium bovis. Clin Vaccine Immunol. 2014 Aug;21(8):1128-36. doi: 10.1128/CVI.00221-14
Species:
Porcine
Sample Type:
Serum (Mycobacterium vaccinated pigs)
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Great productWe used this kit to analyzed cytokine levels in porcine plasma. The kit and protocol were easy to use. RayBiotech completed free slide scanning and data extraction for us.
from University of Pennsylvania,
on
Great productWe used it for porcine heart tissues and the protocols are easy to follow.from UNC,
on
Great productSensitive and robust with good reproducibility. We took advantage of the free scanning services as well. Low price in comparison to others in the market.from University of Memphis,
on
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