Mouse VCAM-1 ELISA Kit
RayBio® Mouse VCAM-1 (CD106) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
|---|---|
| Species | Mouse |
| Accession Number | P29533 |
| Gene Id | 22329 |
| Gene Symbols | VCAM1|VCAM-1 |
| Protein Name / Synonyms | Vascular cell adhesion protein 1 (V-CAM 1) (VCAM-1) (CD antigen CD106) |
| Quantitative/Semi-Quantitative | Quantitative |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1 alpha, MIP-2, MIP-3 beta, RANTES, SCF, sTNFri, TARC, TIMP-1, TNF-alpha, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Solid Support | 96-well Microplate |
| Method Of Detection | Colorimetric |
| Design Principle | Sandwich-based |
| Sensitivity | 1.5 pg/ml Need more sensitivity? Check out the new BIQ-ELISAâ„¢ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoaâ„¢ technology. |
| Detection Range | 1.5 pg/ml - 500 pg/ml |
| Recommended Dilution (Serum/Plasma) | ~200,000 fold |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Burks S., Nguyen B., Tebebi P., et al. Pulsed focused ultrasound pretreatment improves mesenchymal stromal cell efficacy in preventing and rescuing established acute kidney injury in mice. Stem Cells. 2015 Apr;33(4):1241-53. doi: 10.1002/stem.1965.
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Mouse
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Tissue Lysate (Kidneys from Female C3H mice treated with pulsed focused ultrasound from kidney injured mice)
Tebebi P., Burks S., Kim S., et al. Cyclooxygenase-2 or Tumor Necrosis Factor-a Inhibitors Attenuate the Mechanotransductive Effects of Pulsed Focused Ultrasound to Suppress Mesenchymal Stromal Cell Homing to Healthy and Dystrophic Muscle. Stem Cells 2015 Apr;33(4):1173-86. doi: 10.1002/stem.1927
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Mouse
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Schober A., Nazari-Jahantigh M., Wei Y., Bidzhekov K., Gremse F., et al. MicroRNA-126-5p promotes endothelial proliferation and limits atherosclerosis by suppressing Dlk1. Nature Medicine 20, 368-376 (2014)
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Tissue Lysate (Mouse Carotid artery Lysate)
Othman, Azza I., et al. "Epigallocatechin-3-gallate protects against diabetic cardiomyopathy through modulating the cardiometabolic risk factors, oxidative stress, inflammation, cell death and fibrosis in streptozotocin-nicotinamide-induced diabetic rats." Biomedicine & Pharmacotherapy 94 (2017): 362-373.
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Yousef H., Czupalla C., Lee D., et al. Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1. Nat Med . 2019 Jun;25(6):988-1000. doi: 10.1038/s41591-019-0440-4. Epub 2019 May 13.
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Great productThe pre-coated VCAM ELISA kits are highly reproducible and sensitive. Helped me analyze and confirm the VCAM levels that we had detected using the Ray Biotech's AM-CYT-3-8 kit which is a cytokine array kit
from W..Keck Center for Transgene Research,
on
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