RayBio® Mouse TIMP-1 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

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Catalog #:
ELM-TIMP1
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Product Description

Specifications

Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species Mouse
Accession Number P12032
Gene Id 21857
Gene Symbols TIMP1|TIMP|TIMP-1
Protein Name / Synonyms Metalloproteinase inhibitor 1 (Collagenase inhibitor 16C8 fibroblast) (Erythroid-potentiating activity) (EPA) (TPA-S1) (TPA-induced protein) (Tissue inhibitor of metalloproteinases 1) (TIMP-1)
Quantitative/Semi-Quantitative Quantitative
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, Leptin (OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, MCSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Compatible Sample Types Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Solid Support 96-well Microplate
Method Of Detection Colorimetric
Design Principle Sandwich-based
Sensitivity 3 pg/ml

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Detection Range 3 pg/ml - 1000 pg/ml
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage ≤-20°C

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Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Kobayashi, Satoshi, et al. "A single dose of lipopolysaccharide into mice with emphysema mimics human chronic obstructive pulmonary disease exacerbation as assessed by micro-computed tomography." American journal of respiratory cell and molecular biology 49.6 (2013): 971-977.
Species:
Mouse
Sample Type:
BAL Fluid (Mice with Emphysema Mimics Human Chronic Obstructive Pulmonary Disease)
Spruss A., Kanuri G., Stahl C., et al. Metformin protects against the development of fructose-induced steatosis in mice: role of the intestinal barrier function. Lab Invest. 2012 Jul;92(7):1020-32. doi: 10.1038/labinvest.2012.75
Species:
Mouse
Sample Type:
Plasma
Cuaz-Perolin C, Jguirim I, Larigauderie G, Jlassi A, Furman C, et al. Apolipoprotein E Knockout Mice Over-Expression Human Tissue Inhibitor of Metalloproteinase 1 Are Protected against Aneurysm Formation, but Not Against Atherosclerotic Plaque Development. J Vasc Res. 2006;43:493-501.
Species:
Mouse
Sample Type:
Plasma (plasma)
Harland J, Nielsen OL, Brunner N, Offenberg H. Gender-dependent physiological implications of combines PAI-1 and TIMP-1 gene deficiency characterized in a mouse model. Am J Physiol Regul Integr Comp Physiol. 2007; 293:R1630-R1639.
Species:
Mouse
Sample Type:
Plasma (Plasma)
Carroll-Portillo A., Cannon J., Riet J., et al. Mast cells and dendritic cells form synapses that facilitate antigen transfer for T cell activation. J Cell Biol. 2015 Aug 31; 210(5): 851-864. doi: 10.1083/jcb.201412074
Species:
Mouse
Sample Type:
Conditioned Media (Bone marrow derived macrophages)
Harslund, Jakob, et al. "The role of Serpine-1 and tissue inhibitor of metalloproteinase type-1 in early host responses to Staphylococcus aureus intracutaneous infection of mice." Pathogens and disease 68.3 (2013): 96-104.
Species:
Mouse
Sample Type:
Plasma (Staphylococcus aureus intracutaneous infection of mice)
Song J, Hu D, He C, et al. Novel biomarkers for early prediction of sepsis-induced disseminated intravascular coagulation in a mouse cecal ligation and puncture model. J Inflamm (Lond). 2013;10(1):7 doi:10.1186/1476-9255-10-7
Species:
Mouse
Sample Type:
Serum (Serum)
Song J, Hu D, He C, et al. Novel biomarkers for early prediction of sepsis-induced disseminated intravascular coagulation in a mouse cecal ligation and puncture model. J Inflamm (Lond). 2013;10(1):7.
Species:
Mouse
Sample Type:
Serum
Kisarova Y., Korolenko T. Tissue inhibitors of matrix metalloproteinases 1 and 2 and matrix metalloproteinase activity in the serum and lungs of mice with lewis lung carcinoma. Bull Exp Biol Med. 2012 Oct;153(6):874-7.
Species:
Mouse
Sample Type:
Serum (Mice with Lewis lung carcinoma)
Kisarova Y., Korolenko T. Tissue inhibitors of matrix metalloproteinases 1 and 2 and matrix metalloproteinase activity in the serum and lungs of mice with lewis lung carcinoma. Bull Exp Biol Med. 2012 Oct;153(6):874-7.
Species:
Mouse
Sample Type:
Tissue Lysate (Mice with Lewis lung carcinoma, Lung Tissue lysate)
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