Mouse Leptin ELISA Kit
RayBio® Mouse Leptin ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Product Description
Specifications
Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
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Species | Mouse |
Accession Number | P41160 |
Gene Id | 16846 |
Gene Symbols | LEP|OB |
Protein Name / Synonyms | Leptin (Obesity factor) |
Quantitative/Semi-Quantitative | Quantitative |
Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF- 1alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
Solid Support | 96-well Microplate |
Method Of Detection | Colorimetric |
Design Principle | Sandwich-based |
Sensitivity | 4 pg/ml Need more sensitivity? Check out the new BIQ-ELISAâ„¢ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
Detection Range | 4 pg/ml - 1000 pg/ml |
Recommended Dilution (Serum/Plasma) | 10 - 40 fold |
Estimated Lead Time | 1-2 business days |
Shipping Type | Blue ice |
Storage | ≤-20°C |
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Park J., Shim H., Na A., et al. Orexin A regulates plasma insulin and leptin levels in a time-dependent manner following a glucose load in mice. Diabetologia. 2015 Jul;58(7):1542-50. doi: 10.1007/s00125-015-3573-0.
Li E., Shan H., Chen L., et al. OLFR734 Mediates Glucose Metabolism as a Receptor of Asprosin. Cell Metab . 2019 Aug 6;30(2):319-328.e8. doi: 10.1016/j.cmet.2019.05.022. Epub 2019 Jun 20.
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Wang X-A, Zhang R, Jiang D, et al. Interferon regulatory factor 9 protects against hepatic insulin resistance and steatosis in male mice. Hepatology. 2013 Aug;58(2):603-616.
Bader, Manuela I., et al. "Comparative assessment of estrogenic responses with relevance to the metabolic syndrome and to menopausal symptoms in wild-type and aromatase-knockout mice." The Journal of steroid biochemistry and molecular biology 127.3 (2011): 428-434.
Kishino E, Ito T, Fujita K, Kiuchi Y. A mixture of Salacia reticulata (Kotala himbutu) Aqueous Extract and Cyclodextrin Reduces the Accumulation of Visceral Fat Mass in Mice and Rats with High-Fat Diet-Induced Obesity. J Nutr. 2006;136:433-439.
Jiao, Ping, et al. "FFA?Induced Adipocyte Inflammation and Insulin Resistance: Involvement of ER Stress and IKK? Pathways." Obesity 19.3 (2011): 483-491.
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Great productWe recently purchased two mouse Leptin ELM kits and used one so far. we used 5 ul of mouse plasma for the measurement. The mice fed with high fat high sucrose diet and normal chow diet for 12 weeks were used. The leptin level ranged from 2 ng/ml up to 800 ng/ml.
from The University of Iowa,
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