RayBio® L-Series Mouse Antibody Array 308 Glass Slide Kit. Detects 308 Mouse Proteins. Suitable for serum, plasma and cell culture supernatants.

As low as $572.00
In stock
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Catalog #:
AAM-BLG-1
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Product Description

Specifications

Size 4 Sample Kit, 8 Sample Kit
Species Mouse
Quantitative/Semi-Quantitative Semi-Quantitative
Number of Targets Detected 308
Compatible Sample Types Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates
Solid Support Glass Slide
Method Of Detection Fluorescence Laser Scanner
Design Principle Label-based
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage -20°C
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Product Features

  • High density arrays
  • Unbiased detection through direct biotin labeling of samples
  • Low sample consumption
  • High detection sensitivity
  • Same day result
  • Affordable and simple to use

Target Names

6Ckine, Activin A, Activin C, Activin RIB / ALK-4, Adiponectin / Acrp30, AgRP, ALCAM, Angiopoietin-like 2, Angiopoietin-like 3, AREG (Amphiregulin), Artemin, Axl, bFGF, B7-1/CD80, BAFF R / TNFRSF13C, BCMA / TNFRSF17, beta-Catenin, BLC, BTC (Betacellulin), Cardiotrophin-1, CCL1 / I-309 / TCA-3, CCL28, CCL4 / MIP-1 beta, CCL7 / MCP-3 / MARC, CCL8 / MCP-2, CCR10, CCR3, CCR4, CCR6, CCR7, CCR9, CD11b, CD14, CRP, CD27 / TNFRSF7, CD27 Ligand / TNFSF7, CD30, CD30 L, CD40, CD40 Ligand / TNFSF5, Cerberus 1, Chordin-Like 2, Coagulation Factor III / Tissue Factor, Common gamma Chain / IL-2 R gamma, CRG-2, Cripto, Crossveinless-2, Cryptic, Csk, CTACK , CTLA-4 / CD152, CXCL14 / BRAK, CXCL16, CXCR2 / IL-8 RB, CXCR3, CXCR4, CXCR6, DAN, Decorin, DKK-1, Dkk-3, Dkk-4, DPPIV / CD26, DR3 / TNFRSF25, Dtk, EDAR, EGF R, EG-VEGF / PK1, Endocan, Endoglin / CD105, Endostatin, Eotaxin, Eotaxin-2, Epigen, Epiregulin, Erythropoietin (EPO), E-Selectin, FADD, FAM3B, Fas / TNFRSF6, Fas Ligand, FCrRIIB / CD32b, FGF R3, FGF R4, FGF R5 beta, FGF-21, Fit-3 Ligand, FLRG (Follistatin), Follistatin-like 1, Fractalkine, Frizzled-1, Frizzled-6, Frizzled-7, Galectin-3, G-CSF, GDF-1, GDF-3, GDF-5, GDF-8, GDF-9, GFR alpha-2 / GDNF R alpha-2, GFR alpha-3 / GDNF R alpha-3, GFR alpha-4 / GDNF R alpha-4, GITR, GITR Ligand / TNFSF18, Glut2, GM-CSF, Granzyme B, Granzyme D, Granzyme G, Gremlin, Growth Hormone R, HGF R, HGF, HVEM / TNFRSF14, ICAM-1, ICAM-2 / CD102, ICAM-5, ICK, IFN-alpha / beta R1, IFN-alpha / beta R2, IFN-beta, IFN-gamma , IFN-gamma R1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IGFBP-rp1 / IGFBP-7, IGF-I, IGF-II, IL-1 alpha, IL-1 beta, IL-1 R4 / ST2, IL-1 R6 / IL-1 R rp2, IL-1 R9, IL-1 RI, IL-1 RII, IL-2, IL-2 R alpha, IL-2 R beta, IL-3, IL-3 R alpha, IL-3 R beta, IL-4, IL-4 R, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-7 R alpha, IL-9, IL-9 R, IL-10, IL-10 R alpha, IL-11, IL-12 p40/p70, IL-12 p70, IL-12 R beta 1, IL-13, IL-13 R alpha 2, IL-15, IL-15 R alpha, IL-16, IL-17, IL-17B R, IL-17C, IL-17D, IL-17E, IL-17F, IL-17R, IL-17RC, IL-17RD, IL-18 R alpha/IL-1 R5, IL-20, IL-20 R alpha, IL-21, IL-21 R, IL-22, IL-22BP, IL-23, IL-23 R, IL-24, IL-27, IL-28 / IFN-lambda, IL-31, IL-31 RA, Insulin, Integrin beta 2 / CD18, I-TAC, KC, Kremen-1, Kremen-2, Lefty-1, Leptin R, LEPTIN(OB), LIF, LIGHT / TNFSF14, LIX, LRP-6, L-Selectin, Lungkine, Lymphotactin, Lymphotoxin beta R / TNFRSF3, MAdCAM-1, MCP-1, MCP-5, M-CSF, MDC, MFG-E8, MFRP, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 alpha, MIP-3 beta , MMP-2, MMP-3, MMP-9, MMP-12, MMP-14 / LEM-2, MMP-24 / MT5-MMP, Neuregulin-3 / NRG3, Neurturin, NGF R / TNFRSF16, NOV / CCN3, Osteoactivin / GPNMB, Osteopontin, Osteoprotegerin, OX40 Ligand / TNFSF4, PDGF C, PDGF R alpha, PDGF R beta, Pentraxin3 / TSG-14, PF-4, PlGF-2, Progranulin, Prolactin, P-Selectin, RAGE, RANTES, RELM beta, Resistin, S100A10, SCF, SCF R / c-kit, SDF-1, Serum Amyloid A1, Shh-N, SIGIRR, SLPI, Soggy-1, SPARC, Spinesin Ectodomain, TACI / TNFRSF13B, TARC, TCA-3, TCCR / WSX-1, TECK, TFPI, TGF-beta 1, TGF-beta 2, TGF-beta 3, TGF-beta RI / ALK-5, TGF-beta RII, Thrombospondin, Thymus Chemokine-1, Tie-2, TIMP-1 , TIMP-2, TIMP-4, TL1A / TNFSF15, TLR1, TLR2, TLR3, TLR4, TMEFF1 / Tomoregulin-1, TNF RI / TNFRSF1A, TNF RII, TNF-alpha, TNF-beta / TNFSF1B, TPO, TRAIL / TNFSF10, TRAIL R2 / TNFRSF10B, TRANCE / TNFSF11, TREM-1, TROY, TSLP, TSLP R, TWEAK / TNFSF12, TWEAK R / TNFRSF12, Ubiquitin, uPAR, Urokinase, VCAM-1, VE-Cadherin, VEGF, VEGF R1, VEGF R2, VEGF R3, VEGF-B, VEGF-C, VEGF-D, WIF-1, WISP-1 / CCN4

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
  • RayBio® L-Series Antibody Array Glass Slide(s)
  • Spin Columns
  • Labeling Reagent
  • Stop Solution
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Streptavidin-Conjugated Fluor
  • Adhesive Plastic Strips
  • Labeling Buffer
  • Lysis Buffer
  • 30 ml Centrifuge Tube
Other Materials Required
  • Distilled or de-ionized water
  • Small plastic or glass containers
  • Orbital shaker or oscillating rocker
  • 1 ml tube
  • Pipettors, pipette tips and other common lab consumables
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline
  1. Purify Samples with Spin Column
  2. Biotinylate Samples
  3. Purify biotinylated samples using spin column
  4. Dry the glass slide
  5. Block array surface
  6. Incubate with Samples
  7. Incubate with Streptavidin-Conjugated Fluor
  8. Disassemble the glass slide
  9. Scan with a gene microarray laser scanner
  10. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Kano A. Tumor cell secretion of soluble factor(s) for specific immunosuppression. Sci Rep. 2015 Mar 9;5:8913. doi: 10.1038/srep08913.
Species:
Mouse
Sample Type:
Conditioned Media (4T1 conditioned cells)
Guo W., Miller AD., Pencina K., et al. Joint dysfunction and functional decline in middle age myostatin null mice. Bone. 2015 Nov 5;83:141-148. doi: 10.1016/j.bone.2015.11.003. [Epub ahead of print]
Species:
Mouse
Sample Type:
Plasma (Bone remodeling plasma samples)
Ge H., Zhu H., Xu N., et al. Increased Lung Ischemia-Reperfusion Injury in Aquaporin 1-Null Mice Is Mediated via Decreased Hypoxia-Inducible Factor 2? Stability. Am J Respir Cell Mol Biol. 2016 Jun;54(6):882-91. doi: 10.1165/rcmb.2014-0363OC.
Species:
Mouse
Sample Type:
Tissue lysate (Lung Tissue lysates from CD1 strain of mice.)
Tang YL, Zhu WW, Cheng M, Chen LJ, Zhang J, et al. Hypoxic Preconditioning Enhances the Benefit of Cardiac Progenitor Cell Therapy for Treatment of Myocardial Infarction by Inducing CXCR4 Expression. Circ Res. 2009;104:1209-1216. DOI: 10.1161/CIRCRESAHA.109.197723
Species:
Mouse
Sample Type:
Conditioned Media (CLK cells (passages 7-8) 90% confluency in standard media, then transferred to a standard incubator or hypoxia chamber (0.1% O2) and cultured in DMEM containing 0.2% FBS. Twelve hours later, the media were collected, concentrated by usi
Ding Y, Yuan S, Liu X, Mao P, Zhao C, et al. (2014) Protective Effects of Astragaloside IV on db/db Mice with Diabetic Retinopathy. PLoS ONE 9(11):e112207. doi:10.1371/journal.pone.0112207
Species:
Mouse
Sample Type:
Tissue Lysate (Retina lysates)
Zhi-Feng W., Le-Yuan Z., Xia-Hui Z., et al. TLR4-Dependent Immune Response Promotes Radiation-Induced Liver Disease by Changing the Liver Tissue Interstitial Microenvironment during Liver Cancer Radiotherapy. Radiat Res. 2014 Dec;182(6):674-82. doi: 10.1667/RR13630.1.
Species:
Mouse
Sample Type:
Hepatic Fluid (Liver tissue interstitial fluid.)
Yang H., Xia L., Chen J., et al. Stress-glucocorticoid-TSC22D3 axis compromises therapy-induced antitumor immunity. Nat Med . 2019 Sep;25(9):1428-1441. doi: 10.1038/s41591-019-0566-4. Epub 2019 Sep 9.
Species:
Mouse
Sample Type:
Plasma (Stress and anti-tumor immunity model)
  1. Great product
    Rating
    100%
    Our lab has used this array three times for measuring secreted cytokines from macrophages with great results. We have used the testing service since we did not have access to the correct machine to read the slides. Samples were measured quickly with high quality data.

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