Product Features
- High density arrays
- Unbiased detection through direct biotin labeling of samples
- Low sample consumption
- High detection sensitivity
- Same day result
- Affordable and simple to use
Target Names
14-3-3 beta, 14-3-3 zeta, 53BP1, aAmylase, AAT1, ABAT, ABCF1 , ABI3BP, ACAA1, ACAA2, ACACA, ACLY, ACO1, ACTBL2, ACTC1, ACTG1, ACTG2, ACTN1, ADA, ADAMDEC1 , ADAS, ADGRF5, ADGRL4, ADH1, ADH1C, ADH4 , ADH5, ADM, ADP-Ribosyl Cyclase 2, Advillin, AEBP1, AFG3L2 , AGA, Aggrecan, Agrin, AGXT, Ahsp , AIFM1, AKAP9 , AKR1B1, AKR7A2 , ALAD, ALDH16A1 , ALDH1A1, ALDH9A1 , Alkaline Phosphatase, alpha Actinin 4, alpha Tubulin 4, Alpha-synuclein, ALS, Alsin , Aminoacylase 1, Aminopeptidase A, Androgen Receptor, ANGPTL6 , ANGPTL8, Ankrd26, Annexin A1, Annexin A2, Annexin A5, Annexin A6, ANP, ANP32A, Antithrombin III, APLP1, AQR, ARFGEF3, Arp3 , ARPC2, ARPC3, ARPP19, ART3 , ARTS1, ASGR2, ASH2L , ASL, Aspartate Aminotransferase, Aspartyl Aminopeptidase, ASXL1 , ATP5A1, ATPB, B3GNT2, B4GalT1, B7-H2, bad, BASP1, Bassoon , bcl-w, BCoR , beta I Spectrin , beta I Tubulin, beta III Tubulin, BID, BIN2, Biotinidase, BIRC6 , BMP-1, BPGM , BPIFB1, BPIFB2 , Brevican, BRG1, BRSK1, C1QA, C1QB , C1QR, C1RL, C1S, C4BPA , C6, C8A, C8G, C9orf40, CA1, CA150 , CACNB4, Cadherin-22 , Cadherin-6, CALD1, Calpain S1 , Calpastatin, Calponin-2, Calretinin, Calumenin , CAP1 , CAPZA1 , Carbonic Anhydrase II, Carbonic Anhydrase III, Carboxylesterase 1, Carboxypeptidase M, Carboxypeptidase E, Caspase-14, Catalase, Cathelicidin, Cathepsin A, Cathepsin G , Cathepsin H, Cathepsin Z, CBS , CCAR2, CCDC126 , CCDC25, CCS, CD109, CD133, CD148, CD155, CD21, CD39L4, CD41, CD42b, CD48, CD5L, CD98, CDA, CDK2 , CED-6, CENPF, CEP57 , Cezanne , CFB, CFHR1, CFI, CFVII, Chitobiase, Chitotriosidase(1), Cholinesterase, CHORDC1 , CHREBP, Chromogranin B, CKB, CLIC1, CLIP1, CL-P1, CLTA, CNOT1, CO4A2, Coagulation Factor IX, Coagulation Factor V , Coagulation Factor XI, Coagulation Factor XII , Coagulation Factor XIII, Cofilin-1, COG4 , COL19A1 , COL4A3 , Col6A2, COL9A3 , COLEC10 , Collagen I alpha-1, Collagen III , Collagen IV alpha-6 , Collagen IX , Collagen V, Collagen X , Collagen XV , Corneodesmosin, Cortactin, COTL1 , CPB2, CPEB3 , CPNE3 , CRHBP, CrkL(1), CRMP2, CRTAC1, CRYZ, Cyclophilin A, Cyclophilin B, Cystatin, CYTL1, Cytochrome b5, Cytochrome C, Cytokeratin 1, Cytokeratin 10, Cytokeratin 13, Cytokeratin 14, Cytokeratin 15, Cytokeratin 20 , Cytokeratin 9 , D4, DAN, DARS2 , DBH, DCXR, DDAH1, DDT, DDX3Y, DEFA6, Desmocollin 1, Desmocollin-2, Desmocollin-3, Desmoglein-1, Desmoglein-2, Desmoplakin 3, DGK-theta, DISC 1, DMRN9, DOT1L, DPP3, DRIL1 , DSCAM, DSPG3, ECHS1 , ECI1, ECM-1, EEF1G , EEF2, EFEMP2 , EFTUD2, EHD3 , Eif4a1, ELAVL1 , EMSY , EN2 , Endorepellin, ENO3, ENSA , EPB41, EPCR, Ephrin-B1, Eps 15, ERAB, ERp29, ERp57, ERp72, ESD, ESR1, Ezrin, FABP5, FAH, FAM20C , FAM3C, FASN, FASTKD5, FBP 38 , FDPS , FGG, Fibrillin 1, Fibrinogen-like 2, Fibrinopeptide B, Fibulin 3, Ficolin 2, Filamin C, FKBP1A, FKBP25, FKBP51, Fodrin alpha chain, Frizzled 8 , FRY, FSH-B, FTL1, FUCA2, FUS , G3BP1, G6PD, GALNT2, GANAB, GAPHD, GARNL1 , GART, Gastrokine 1, GATM, GBE1, GCDFP 15, GCLC , GCSH , GDA, GDF7 , GDI1, GDI2, Gephyrin, GFAP, GGCT, GGH, GIP, GLIPR2, GLUD1 , Glycoprotein V, GM2A, GMF beta, GNB1 , GNPTG, GOLIM4, GOLM1, GPD1, GPLD1, GRHPR, GRP170, GSS, GSTM1, GSTO1, GSTP1, Guanylin, GZMM, H6PD, HABP2, HBB, HDGF, Hemoglobin, Hemoglobin A1c, HEXB, HGFA, HIBADH , HINT1, HIP1R , Histone H1.2 , Histone H1.4, Histone H2A , Histone H2A.Z, Histone H2B K, Histone H3.3 , Histone H4 , HMGB1, HMGB2 , HMGB3, HMGN2, HNF-3 alpha, hnRNP A1 , hnRNP A2B1 , hnRNP C1 + C2 , hnRNP G , hnRNP L , hnRNP M , hnRNP U , Hornerin, Hoxb3 , HOXD11 , HP1BP3, HPD, HPRG, HPRT1, HRP12, HSPA1A, HTRA1, HUWE1, IDH1, IFRD1 , IGFBP-rp1, IGSF4B, ILK, IMP2/IGF2BP2/p62, Inhibin beta , Integrin alpha-6, Integrin b1, Integrin beta-6, IQGAP2 , IRE1, IRS2 , ISOC2, ITGB4BP, ITIH2, ITIH3, ITIHC4, JAM-A, JPT1, KDM4B, Keratin 36, KIAA0319L, KIAA1468, KLKB1, KMT2D, KRT31, KRT33B, KRT73, KRT82, KRT85 - N-terminal, KSR1 , LAF4, LAIR1, LAM b1, Lamin A/C, Lamin B2 , Laminin 2 alpha, Laminin b2 , Laminin gamma 1, LAMP, LAMP1, LASP1 , LCAT , LCMT2 , LDH-H, LEDGF, Limbin, LIMS1, LMW-PTP, LOK , LOX , LOXL1, LPA, LTBP4, Lubricin, LUZP1 , LYZL1, MAGI2, MAN1 , MAN1A1, Mannosidase II , MAP1A , MAPRE1 , MARCKS, MASP3, MBD2 , MBP, MCAM, Mcl-1, MCM, MDH1 , Meprin alpha, Metallothionein 2, Metavinculin, MFAP4, MFI2, mGLUR5, Mimecan, MLCK, MMR, MN1 , Moesin, MP1, MPCA, MRP 1, MSH6, Mtor, Multimerin 2, MyBPC3, Myeloperoxidase, MYH2 , MYH6, MYH7, MYHC 2x, MYL12B , MYO5A , Myoferlin , Myosin 18B , Myosin9, NABC1 , NAGLU, NAP1L1 , NAPRT1, NASP , NCAM2 , Nebulin , Nectin-1, Nectin-3, Neogenin, Nesprin2, Neurofibromin , Neurogranin, Neuropeptide B, Neuropilin-1, Neurotrimin, NF-M, NIF3L1, NME3 , nNOS1, Notch-2, NPAS3 , NPM1, Thrombospondin 5
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
- RayBio® L-Series Antibody Array Glass Slide(s)
- Spin Columns
- Labeling Reagent
- Stop Solution
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Streptavidin-Conjugated Fluor
- Adhesive Plastic Strips
- Labeling Buffer
- Lysis Buffer
- 30 ml Centrifuge Tube
Other Materials Required
- Distilled or de-ionized water
- Small plastic or glass containers
- Orbital shaker or oscillating rocker
- 1 ml tube
- Pipettors, pipette tips and other common lab consumables
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Purify Samples with Spin Column
- Biotinylate Samples
- Purify biotinylated samples using spin column
- Dry the glass slide
- Block array surface
- Incubate with Samples
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.