Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species	Mouse
Accession Number	P10749
Gene Id	16176
Gene Symbols	IL1B
Protein Name / Synonyms	Interleukin-1 beta (IL-1 beta)
Quantitative/Semi-Quantitative	Quantitative
Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Solid Support	96-well Microplate
Method Of Detection	Colorimetric
Design Principle	Sandwich-based
Sensitivity	5 pg/ml Need more sensitivity? Check out the new BIQ-ELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	5 pg/ml - 2000 pg/ml
Recommended Dilution (Serum/Plasma)	2 fold
Estimated Lead Time	1-2 business days
Shipping Type	Blue ice
Storage	≤-20°C

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Target Background

Interleukin-1β (IL1b) is a pro-inflammatory cytokine important for immune responses to infection and injury. It is produced as a 31 kDa precursor, termed pro-IL-1b which is cleaved by caspase-1-dependent processing for secretion. IL1b is expressed in a wide variety of tissues and cells, especially in macrophages in the thymus, spleen, lymph nodes, Peyer's patches and bone marrow. Disruption of IL1b signaling can lead to inflammatory disease and malignancies.

Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Documents

Datasheet (ELM-IL1b.pdf)

Datasheet (ELM-IL1b-CL.pdf)

SDS (ELM-IL1b.pdf)

SDS (ELM-IL1b-CL.pdf)

Excel (sandwich-elisa.xlsm)

Citations

Vazquez C., Tolon R., Grande M., et al. Endocannabinoid regulation of amyloid-induced neuroinflammation. Neurobiol Aging. 2015 Nov;36(11):3008-19. doi: 10.1016/j.neurobiolaging.2015.08.003.

Species:

Mouse

Sample Type:

Tissue Lysate (Hippocampal lysate )

View Publication

Morganti JM, Nash KR, Grimmig BA, et al. The soluble isoform of CX3CL1 is necessary for neuroprotection in a mouse model of Parkinson's disease. The Journal of neuroscience?: the official journal of the Society for Neuroscience 2012;32(42):14592-14601. doi:10.1523/JNEUROSCI.0539-12.2012.

Species:

Mouse

Sample Type:

Tissue Lysate (Brain Lysate, ventral mesencephalon)

View Publication

Ge, J.-C., Zha, X.-Q., Nie, C.-Y., Yu, N.-J., Li, Q.-M., Peng, D.-Y., Luo, J.-P. (2018). Polysaccharides from Dendrobium huoshanense stems alleviates lung inflammation in cigarette smoke-induced mice. Carbohydrate Polymers, 189, 289-295. doi:10.1016/j.carbpol.2018.02.055

Species:

Mouse

Sample Type:

Serum

View Publication

Zhang Y., et al. Interferon regulatory factor 9 is an essential mediator of heartdysfunction and cell death following myocardial ischemia/reperfusion injury. Basic Res Cardiol (2014) 109:434. DOI 10.1007/s00395-014-0434-9

Species:

Mouse

Sample Type:

Tissue Lysate (Mouse ischemic heart tissue)

View Publication

Zhang X. et al. TRAF1 is a key mediator for hepatic ischemia/reperfusion injury. Cell Death and Disease (2014) 5, e1467; doi:10.1038/cddis.2014.411

Species:

Mouse

Sample Type:

Serum (Hepatic Injury samples)

View Publication

Liu G, Zhang H, Hao F, Hao J, Pan L, Zhao Q, Wo J: Clusterin Reduces Cold Ischemia-Reperfusion Injury in Heart Transplantation Through Regulation of NF-kB Signaling and Bax/Bcl-xL Expression. Cell Physiol Biochem 2018;45:1003-1012. doi: 10.1159/000487296

Species:

Mouse

Sample Type:

Conditioned Media (heart tissue)

View Publication

Ding W, Liu T, Bi X, Zhang Z: Mitochondria-Targeted Antioxidant Mito-Tempo Protects Against Aldosterone-Induced Renal Injury In Vivo. Cell Physiol Biochem 2017;44:741-750. doi: 10.1159/000485287

Species:

Mouse

Sample Type:

Plasma

View Publication

Yu L., et al. MRTF-A mediates LPS-induced pro-inflammatory transcription by interacting with the COMPASS complex. Journal of Cell Science. Advance Online Article September 4, 2014 doi: 10.1242/jcs.152314

Species:

Mouse

Sample Type:

Conditioned Media

View Publication

Yu L., et al. MRTF-A mediates LPS-induced pro-inflammatory transcription by interacting with the COMPASS complex. Journal of Cell Science. Advance Online Article September 4, 2014 doi: 10.1242/jcs.152314

Species:

Mouse

Sample Type:

Tissue Lysate (Intestinal tissue from DSS colitis)

View Publication

Ho P., Tsui Y., Feng X., et al. NF-?B-mediated degradation of the coactivator RIP140 regulates inflammatory responses and contributes to endotoxin tolerance. Nat Immunol. 2012 Mar 4;13(4):379-86. doi: 10.1038/ni.2238.

Species:

Mouse

Sample Type:

Conditioned Media (Raw264.7 macrophages with LPS treatement)

View Publication

View All Publications For This Product

Reviews

Write a customer review

Finally! A tissue homogenate-compatible IL-1b ELISA!

Rating
100%

We've been looking for an ELISA kit that detects IL-1b in tissue lysates for ages. We used this kit with mouse brain tissue homogenized in a lysis buffer containing 0.25% SDS and diluted the samples 1:5 as recommended by the manufacturer protocol and got beautiful results.

Review by Felecia,

from University of Illinois at Chicago,

on Jul 26, 2024
Easy to use this kit

Rating
100%

I recently had the opportunity to use the IL-1β ELISA kit for quantifying interleukin-1 beta (IL-1β) levels in mouse skin tissue, and I am extremely pleased with the results. This kit proved to be both user-friendly and reliable, making it an excellent choice for researchers studying inflammation and cytokine expression.

Review by Ayaz,

from Western University of Health Science,

on Jul 21, 2024
Great product

Rating
100%

I measured the Mouse IL-1 beta (Cat#Ray Bio Mouse IL-1 Beta ELISA KIT) in Kidney tissue homogenates from mouse model of Diabetes (DB/DB-UNX) with Uni nephrectomy (UNX). I see the increased IL-1B levels in DB/DB-UNX compared to control Non-Diabetic (DB/M) mice. In this models we knew that there is increased inflammation together with Inflammasome signalling in the kidney of DB/DB-UNX. Therefore, I am interested in measure the IL-1B in our mouse of model of Diabetic kidney disease. This Kit from RayBiotech is working great in our mouse model and I would recommend this product.

Review by Komuraiah Myakala,

from Georgetown University,

on Jan 23, 2022

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