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Mouse Cytokine Array Q400
Quantibody® Mouse Cytokine Array 400 Kit. A combination of 10 non-overlapping arrays to quantitatively measure 400 Mouse cytokines.
Product Description
Specifications
Size
8 Sample Kit, 22 Sample Kit, 50 Sample Kit
Species
Mouse
Quantitative/Semi-Quantitative
Quantitative
Number of Targets Detected
400
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Solid Support
Glass Slide
Method Of Detection
Fluorescence Laser Scanner
Design Principle
Sandwich-based
Shipping Type
Blue ice
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Product Features
More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation
Target Names
2B4 (CD244), 4-1BB (TNFRSF9/CD137), 4-1BB Ligand (TNFSF9), 6Ckine (CCL21), ACE (CD143), ACE-2, Activin A, Activin R1B (ALK-4), ADAM15, ADAM-9, ADAMTS-1 (METH1), Adiponectin (ACRP30), ADP-Ribosyl Cyclase 2 (BST-1/CD157), ALK-1, Alpha-fetoprotein, Ameloblastin, Amphiregulin, ANGPTL1, ANGPTL3, ANGPTL7, Artemin, ASAHL, ASAM (ACAM), AXL, B7-H2 (ICOS Ligand), B7-H3 (CD276), B7-H4, BAFF, BAFF R, Betacellulin (BTC), bFGF, BLC (CXCL13), C1q R1 (CD93), C4.4A (LYPD3), C5a, CA12, CA9, Cadherin-4, Carbonic Anhydrase IV (CA4), Carbonic Anhydrase XIV (CA14), Cardiotrophin-1 (CT-1), Cathepsin H, CCL28 (MEC), CCL6, CD117 (c-kit), CD14, CD2, CD200, CD229 (SLAMF3), CD27 (TNFRSF7), CD27 Ligand (TNFSF7), CD28, CD30 (TNFRSF8), CD30 Ligand (TNFSF8), CD300b (LMIR5), CD32a, CD36 (SR-B3), CD39 (ENTPD1), CD39L3 (ENTPD3), CD4, CD40 (TNFRSF5), CD40 Ligand (TNFSF5), CD44, CD45, CD48 (SLAMF2), CD6, CD69, CD80 (B7-1), CD86 (B7-2), CD90 (Thy1), CD99-L2, CDCP1, CDNF, CEACAM-1, CFVII, Chemerin, CHL-1 (L1CAM-2), Chordin, Chordin-like 2 (CHRDL2), CLEC9A, Clusterin, COCO, Contactin-4 (BIG-2), Contactin-6, CRACC (SLAMF7), Cripto-1, CRP (C-Reactive Protein), CTLA-4 (CD152), CXADR, CXCL14 (BRAK), CXCL16, Cystatin B, Cystatin C, DAN, DcTRAIL R1 (TNFRSF23), Decorin, Dectin-2 (CLEC6A), DKK-1, DLL1, DLL4, DNAM-1 (CD226), DNER, Dtk, E-Cadherin, EDAR, EGF, Endocan, Endoglin (CD105), Endoglycan (PODXL2), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), EphA5, EphA8, EphB2, EphB4, EphB6, Ephrin-A2, Ephrin-A4, Epigen, Epimorphin (Syntaxin 2), Epiregulin, Erythropoietin R, E-Selectin, Fas (TNFRSF6/Apo-1), Fas Ligand (TNFSF6), FCRL1 (FcRH1), Fetuin A, FGF-10 (KGF-2), FGF-21, FGF-4, FGF-6, FLRG, Flt-3 (Flk-2), Flt-3 Ligand, Follistatin, Follistatin-like 1, Fractalkine (CX3CL1), Frizzled-1, Frizzled-4, Galectin-1, Galectin-3, Galectin-7, Gas 1, Gas 6, GCSF, GCSF R (CD114), GDF-3, GDF-7 (BMP-12), GFR alpha-3, GITR (TNFRSF18), GITR Ligand (TNFSF18), GM-CSF, gp130 (sgp130), GPV (CD42d), GPVI, Granzyme B, Gremlin-1, GRO alpha (CXCL1), H60, HAI-1, Heparan Sulfate 6-O-Sulfotransferase 3 (HS6ST3), HGF, HGFR, Histone Deacetylase 8 (HDAC8), I-309 (TCA-3/CCL1), ICAM-1 (CD54), IFN-gamma, IFN-gamma R1, IFN-gamma R2, IGF-1, IGF-2, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IGSF8 (CD316), IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 R1, IL-1 R2, IL-1 R4 (ST2), IL-1 R6 (IL-1 Rrp2), IL-1 Ra (IL-1 F3), IL-10, IL-10 R beta, IL-11 R alpha, IL-12 p40/p70, IL-12 p70, IL-13, IL-15, IL-15 R alpha, IL-16, IL-17 RA, IL-17 RB, IL-17 RC, IL-17A, IL-17B, IL-17C, IL-17E (IL-25), IL-17F, IL-18 BPc, IL-18 R beta (AcPL), IL-2, IL-2 R alpha, IL-20, IL-20 R beta, IL-21, IL-21 R, IL-22, IL-23, IL-23 R, IL-28A (IFN-lambda 2), IL-28A (IFN-lambda 2), IL-3, IL-3 R beta, IL-31, IL-33 (IL-1 F11), IL-34, IL-36 beta (IL-1 F8), IL-4, IL-5, IL-6, IL-7, IL-7 R alpha (CD127), IL-9, I-TAC (CXCL11), JAM-A (CD321/F11R), JAM-C (CD323), Kallikrein 7, Klotho beta, Kremen-1, Kremen-2, Laminin alpha 4, LAMP1 (CD107a), Layilin, LDL R, Leptin, Leptin R, LIF, LIGHT (TNFSF14), Limitin, LIMPII, Lipocalin-2 (NGAL), LIX, LOX-1, LRPAP, LRRC32 (GARP), L-Selectin (CD62L), Lungkine (CXCL15), Lymphotactin (XCL1), Lymphotoxin beta R (TNF RIII), MAdCAM-1, Marapsin, Mast Cell Protease-11 (Prss34), Mast Cell Protease-6 (Mcpt6), Matrilin-2, Matrilin-3, MBL-2, MCP-1 (CCL2), MCP-5, M-CSF, MDC (CCL22), MEP1A, MEPE (OF45), MESDC2, Meteorin, Meteorin-like (METRNL), MFG-E8, MIG (CXCL9), Mimecan (Osteoglycin), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 gamma, MIP-2, MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MMP-10, MMP-2, MMP-3, Nectin-2 (CD112), Nephrin, Neprilysin, Neurocan, Neurturin, NGFR (TNFRSF16), NgR, NKp46 (NCR1), Nope, NOV (CCN3), Olfactomedin-1, Oncostatin M, Oncostatin M R beta (IL-31 R beta), Osteoactivin (GPNMB), Osteoadherin (Osteomodulin), Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), OX40 (TNFRSF4), OX40 Ligand (TNFSF4), P-Cadherin, PD-1, PDGF R beta, PDGF-AA, PD-L2 (B7-DC), Pentraxin-3 (TSG-14), Periostin, Persephin, PILR-beta, PLA2G2A, Platelet Factor 4 (CXCL4), Plexin A1, Plexin C1, PLGF-2, Podocalyxin, Podoplanin, Progranulin, Prolactin, Pro-MMP-9, Prostasin, Protocadherin-12, P-Selectin, RAGE, RANK (TNFRSF11A), RANTES (CCL5), Reg2, Relaxin-1, Renin 1, Resistin, Ret, RGM-B, RGM-C (Hemojuvelin), ROBO3, SCF, SDF-1 alpha (CXCL12 alpha), SEMA3F, Semaphorin 3C, Semaphorin 4C, Semaphorin 4F, Semaphorin 4G, Semaphorin 6C, sFRP-3, Siglec-3, Siglec-E, SIGNR1 (CD209b), SLAM (CD150), Slit2, SMOC-1, Sonic Hedgehog N-Terminal (Shh-N), SorCS2, SP-D, SR-AI (MSR), STC-2, Syndecan-1, Syndecan-3, TACI (TNFRSF13B), TARC (CCL17), TCK-1 (CXCL7), TECK (CCL25), Testican 3 (SPOCK3), TFPI-2, TGF beta 1, TGF-beta RI (ALK-5), TGF-beta RII, Thrombopoietin (TPO), Thrombopoietin R (Tpo R), TIGIT, TIM-1 (KIM-1), TIM-3 (KIM-3), TLR2, TNF alpha, TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TNFRH3 (TNFRSF26), TRAIL (TNFSF10), TRAIL R2 (TNFRSF10B/DR5), TRANCE (TNFSF11), Transglutaminase 2 (TGM2), TREM-1, TREM-2, TREML1 (TLT-1), TRKC, TROP1 (EpCAM), TROP-2, TROY (TNFRSF19), Trypsin 3 (PRSS3), Tryptase epsilon, Tryptase-5, TSLP, TSLP R, TWEAK (TNFSF12), TWEAK R (TNFRSF12), uPAR, VCAM-1 (CD106), VE-Cadherin (CDH5), VEGF-A, VEGF-B, VEGF-D, VEGFR1, VEGFR2, VEGFR3, Wnt-2bMouse
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
Mouse Cytokine Array Q400 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
30 ml Centrifuge Tube
Adhesive Plastic Strips
Manual
Other Materials Required
Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays!
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Protocol Outline
Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
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