Quantibody® Mouse Chemokine Array 1 Kit. Detects 25 Mouse Chemokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
|---|---|
| Species | Mouse |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 25 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
|
6Ckine
(CCL21)
|
BLC
(CXCL13)
|
CTACK
(CCL27)
|
CXCL16
|
Eotaxin-1
(CCL11)
|
|
Eotaxin-2
(MPIF-2/CCL24)
|
Fractalkine
(CX3CL1)
|
I-309
(TCA-3/CCL1)
|
I-TAC
(CXCL11)
|
KC
(CXCL1)
|
|
LIX
|
MCP-1
(CCL2)
|
MCP-5
|
MDC
(CCL22)
|
MIG
(CXCL9)
|
|
MIP-1 alpha
(CCL3)
|
MIP-1 gamma
|
MIP-2
|
MIP-3 alpha
(CCL20)
|
MIP-3 beta
(CCL19)
|
|
Platelet Factor 4
(CXCL4)
|
RANTES
(CCL5)
|
SDF-1 alpha
(CXCL12 alpha)
|
TARC
(CCL17)
|
TECK
(CCL25)
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Mouse Chemokine Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Lança T, Costa MF, Gonçalves-Sousa N, et al. Protective Role of the Inflammatory CCR2/CCL2 Chemokine Pathway through Recruitment of Type 1 Cytotoxic ?? T Lymphocytes to Tumor Beds. J Immunol. 2013 May 17;
Species:
Mouse
Sample Type:
Tissue Lysate (Tumor lysate from B16-F0 cells)
Ye B., Deng X., Shao L., et al. Vibsanin B preferentially targets HSP90?, inhibits interstitial leukocyte migration, and ameliorates experimental autoimmune encephalomyelitis. J Immunol. 2015 May 1;194(9):4489-97. doi: 10.4049/jimmunol.1402798
Species:
Mouse
Sample Type:
Tissue Lysate (Mouse Spinal cords during encephalomyelitis)
Scanlon S., Thomas S., Ferreira C., Bai L., et al. Airborne lipid antigens mobilize resident intravascular NKT cells to induce allergic airway inflammation. J Exp Med. 2011 Sep 26;208(10):2113-24. doi: 10.1084/jem.20110522
Species:
Mouse
Sample Type:
Tissue Lysate (Lung Tissue Lysate)
Oyebamiji, A. I., Finlay, T. M., Hough, R. M., Hoghooghi, V., Lim, E. M. F., Wong, C. H., & Ousman, S. S. (2013). Characterization of migration parameters on peripheral and central nervous system T cells following treatment of experimental allergic encephalomyelitis with CRYAB. Journal of neuroimmunology, 259(1), 66-74.
Species:
Mouse
Sample Type:
Conditioned Media (cultured spinal cords)
Chen, Xu, et al. "Mononuclear-macrophages but not neutrophils act as major infiltrating anti-leptospiral phagocytes during leptospirosis." PLoS One 12.7 (2017): e0181014.
Species:
Mouse
Sample Type:
Serum
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Great productI've ordered the sample kit numerous times. If you can follow directions, the protocol was easy. I have no issues.
from NIH,
on
Great productIt is the only ELISA Array that gives a quantified assessment of 25 chemokines in one go.protocol is easy to follow and with Ray-biotech providing free scanning of slides, it is the best chemokine ELISA Array available.from NIH,
on
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