Anti-SARS-CoV-2 S1RBD Antibody, Biotinylated
Product Description
Specifications
| Size | 50 ug |
|---|---|
| Host Species | Mouse |
| Accession Number | QHD43416 |
| Protein Name / Synonyms | SARS-CoV-2 S1RBD |
| Clonality | Monoclonal |
| Clone | 1F9-F2-C1-G9 |
| Immunogen | Immunogen was recombinant SARS-CoV-2, S1 Subunit Protein (RBD) with C-terminal His tag, derived from the transfected human HEK293 cells. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by thiophilic medium with 2-mercaptopyridine. |
| Recommended Applications | ELISA (recommended work dilution = 1:320,000) Neutralization was measured by ability to inhibit entry of pseudovirus harboring the SARS-CoV-2 spike protein into A549 cells. This antibody was also demonstrated to block S1 RBD-ACE2 binding in vitro. |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in 1 x PBS. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
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Description
It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Storage
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.