Immunogen was recombinant protein derived from human Tau. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography.
Isotype
IgG1
Recommended Applications
ELISA (recommended work dilution= 1:1,000-4,000), Western Blotting( recommended work dilution= 2-5 µg/ml), Note: Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
Reconstitution
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
Microtubule-associated protein, tau is abnormally hyperphosphorylated in the brain of patients with Alzheimer's disease, and is the major protein subunit of paired helical filaments. There is also a significant pool of non-paired helical filament abnormally phosphorylated tau in Alzheimer's disease brain. Tau is a family of six isoforms, derived from a single gene by mRNA splicing. Tau protein is produced by a single gene expressed predominantly in neurons. They vary in size from 352-441 amino acides. In Alzheimer disease Tau is hyperphosphorylated, containing 3-4 fold more phosphoate/mole of the protein than the normal tau. Recent investigations show that MARK and PKA phosphorylate several sites within the repeats (notably the KXGS motifs including Ser262, Ser324, and Ser356, plus Ser320); in addition PKA phosphorylates some sites in the flanking domains, notably Ser214. This type of phosphorylation strongly reduces tau's affinity for microtubules, and at the same time inhibits tau's assembly into PHFs (Paired helical filament).
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The antibody specifically detected the Tau protein derived from human brain tissue in both ELISA and WB. This antibody showed no cross-reactivity with other unrelated protein.
Related Products
Rabbit anti Tau(Ser262), Cat#RB-02-0013 ; Mouse anti Tau(pThr231), Cat#RB-02-0021; Rabbit anti Tau(Ser396), Cat#RB-02-0014; Mouse anti Tau(pSer199), Cat#RB-02-0022
References
Gong CX, Grundke-Iqbal I, Iqbal K(1994).Dephosphorylation of Alzheimer's disease abnormally phosphorylated tau by protein phosphatase-2A.Neuroscience. 61(4):765-72.
Biernat, J., et al. (1992). The switch of tau protein to an Alzheimer-like state includes the phosphorylation of two serine-proline motifs upstream of the microtubule binding region. EMBO J. 11(4), 1593-1597.
Expiration:
12 months from the date of shipment when stored properly.
