Immunogen was recombinant protein. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography.
Recommended Applications
ELISA (recommended work dilution= 1:640,000), Western Blotting (recommended work dilution= 1:1,000-5,000),
Interleukin-10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, IL-10 is encoded by the IL10 gene.
Specificity
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat IL-10 has not been tested yet.
Storage
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquotted and stored at 4°C for 1 month or at -20°C to -80°C in a manual defrost freezer for many months without detectable loss activity.
Expiration
12 months from the date of shipment when stored properly.